Localization and quantification of endoplasmic reticulum Ca(2+)-ATPase isoform transcripts.
Localization and quantification of endoplasmic reticulum Ca(2+)-ATPase isoform transcripts.
复制标题
内质网 Ca(2)-ATPase 亚型转录物的定位和定量。
DOI：
10.1152/ajpcell.1995.269.3.c775
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Lytton,J
中科院分区：
文献类型：
--
作者：
Wu,KD;Lee,WS;Wey,J;Bungard,D;Lytton,J
肌浆网或内质网Ca（2+）-腺苷三磷酸酶泵（SERCA）在所有细胞的Ca 2+信号传导和稳态中起关键作用，并且由同源和选择性剪接基因家族编码。为了更清楚地了解不同亚型在细胞生理学中的作用，我们对编码每个SERCA亚型的转录本的组织分布进行了定量和定性评估。SERCA 1的表达仅限于快收缩横纹肌，SERCA 2a仅限于心肌和慢收缩横纹肌，而SERCA 2b广泛表达。SERCA 3在大肠和小肠、胸腺和小脑中表达最丰富，在脾脏、淋巴结和肺中表达水平较低。原位杂交分析显示，SERCA 3的成绩单细胞的肠隐窝，胸腺皮质，小脑浦肯野细胞。此外，SERCA 3在分离的大鼠脾淋巴细胞、各种小鼠淋巴细胞系和原代培养的微血管内皮细胞中大量表达。该分析表明，SERCA 3选择性地在细胞中表达，其中Ca 2+信号传导在调节生理过程中起着关键和敏感的作用。
The Ca(2+)-adenosinetriphosphatase pump of the sarcoplasmic or endoplasmic reticulum (SERCA) plays a critical role in Ca2+ signaling and homeostasis in all cells and is encoded by a family of homologous and alternatively spliced genes. To understand more clearly the role the different isoforms play in cell physiology, we have undertaken a quantitative and qualitative assessment of the tissue distribution of transcripts encoding each SERCA isoform. SERCA1 expression is restricted to fast-twitch striated muscles, SERCA2a to cardiac and slow-twitch striated muscles, whereas SERCA2b is ubiquitously expressed. SERCA3 is expressed most abundantly in large and small intestine, thymus, and cerebellum and at lower levels in spleen, lymph node, and lung. In situ hybridization analyses revealed SERCA3 transcripts in cells of the intestinal crypt, the thymic cortex, and Purkinje cells in cerebellum. In addition, SERCA3 was expressed abundantly in isolated rat spleen lymphocytes, in various murine lymphoid cell lines, and in primary cultured microvascular endothelial cells. This analysis demonstrates that SERCA3 is expressed selectively in cells in which Ca2+ signaling plays a critical and sensitive role in regulating physiological processes.
影响因子：
18.2
作者：
M. Lipkin
通讯作者：
M. Lipkin
I 型碘甲状腺原氨酸 5-脱碘酶信使核糖核酸定位于大鼠肾近端小管的 S3 段。
影响因子：
4.8
作者：
Lee,WS;Berry,MJ;Hediger,MA;Larsen,PR
通讯作者：
Larsen,PR
影响因子：
56.9
作者：
David W. Tank;M. Sugimori;John A. Connor;R. R. Llinás-R.
通讯作者：
David W. Tank;M. Sugimori;John A. Connor;R. R. Llinás-R.