Genome-wide identification and characterization of sweet orange (Citrus sinensis) aquaporin genes and their expression in two citrus cultivars differing in drought tolerance
Genome-wide identification and characterization of sweet orange (Citrus sinensis) aquaporin genes and their expression in two citrus cultivars differing in drought tolerance
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甜橙（Citrus sinensis）水通道蛋白基因的全基因组鉴定和表征及其在两个耐旱性不同的柑橘品种中的表达
DOI：
10.1007/s11295-019-1321-1
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发表时间：
2019-04
影响因子：
2.4
通讯作者：
Qingqing Gu
中科院分区：
文献类型：
--
作者：
Qingjiang Wei;Qiaoli Ma;Zhangzheng Ma;Gaofeng Zhou;Fangfang Feng;Si Le;Changyu Lei;Qingqing Gu
水通道蛋白（AQPs）是一个高度保守的主要内在蛋白（MIPs）超家族，在调节水和其他小分子跨膜运动中起关键作用。然而，关于柑橘AQP基因家族的信息有限。在这里，我们对甜橙AQP的同源基因进行了全基因组搜索，并鉴定出34个全长AQP基因（CsAQPs），这些基因位于所有9条染色体上。系统发育分析表明，CsAQPs可分为5个亚家族，包括11个质膜内在蛋白（PIPs）、9个tono质体内在蛋白（TIPs）、8个nod26样内在蛋白（NIPs）、3个小碱性内在蛋白（SIPs）和3个X内在蛋白（XIPs）。基因结构在每个亚家族内普遍保守，内含子数从0到4不等。基于NPA基序、芳香/精氨酸（ar/R）选择性过滤器、Froger位置和特异性决定位置（sdp）分析的功能预测显示，亚科间底物特异性存在显著差异。此外，对干旱处理下耐旱品种（HJ）和干旱敏感品种（HH）根系和叶片中csaqp基因的转录谱分析显示，干旱处理下两种品种的根中cspipps和cstips2基因大部分下调。此外，cspip1;2、CsTIP3；2、andCsNIP2;1,CsNIP1;3,CsNIP4；1、andCsNIP5;1英寸叶片在耐旱和敏感品种间表现出明显的差异。总的来说，这些发现提供了有价值的知识，进一步了解AQP基因在柑橘抗旱性中的潜在生物学功能。
Aquaporins (AQPs), which belong to a highly conserved superfamily of major intrinsic proteins (MIPs), play key roles in regulation of movement of water and other small molecules across membranes. However, information concerning the AQP gene family in citrus is limited. Here, we conducted a genome-wide search for the homologs of AQPs in sweet orange and identified 34 full-length AQP genes (CsAQPs) that were located on all nine chromosomes. Phylogenetic analysis revealed that the CsAQPs could be classified into five subfamilies, including 11 plasma membrane intrinsic proteins (PIPs), nine tonoplast intrinsic proteins (TIPs), eight NOD26-like intrinsic proteins (NIPs), three small basic intrinsic proteins (SIPs), and three X intrinsic proteins (XIPs). Gene structure was generally conserved within each subfamily, with intron numbers ranging from zero to four. Functional prediction based on the analysis of the NPA motifs, aromatic/arginine (ar/R) selectivity filter, Froger’s positions, and specificity-determining positions (SDPs) revealed remarkable differences in substrate specificity among subfamilies. Furthermore, analysis of the transcription profile ofCsAQPgenes in the roots and leaves of drought-tolerant (HJ) and drought-sensitive (HH) cultivars under drought treatment revealed that mostCsPIPsandCsTIPswere down-regulated in roots of both treated cultivars. In addition, the down-regulation ofCsPIP1;2,CsTIP3;2, andCsNIP2;1in roots and up-regulation ofCsNIP1;1,CsNIP1;3,CsNIP4;1, andCsNIP5;1in leaves revealed obvious differences between tolerant and sensitive cultivars during drought. Collectively, these findings provide valuable knowledge that furthers our understanding of the potential biological functions of AQP genes in drought tolerance of citrus.
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影响因子：
5.6
作者：
Rodrigues MI;Takeda AA;Bravo JP;Maia IG
通讯作者：
Maia IG
影响因子：
6.9
作者：
Lin, Wuling;Peng, Yanhui;Cai, Weiming
通讯作者：
Cai, Weiming
麻风树水通道蛋白基因的全基因组鉴定和比较分析为巴西橡胶树基因家族的扩展和进化提供了见解
影响因子：
5.6
作者：
Zou Z;Yang L;Gong J;Mo Y;Wang J;Cao J;An F;Xie G
通讯作者：
Xie G
DOI：
10.1071/fp09117
发表时间：
2009-12
期刊：
Functional plant biology : FPB
影响因子：
--
作者：
M. Shelden;S. Howitt;B. Kaiser;S. Tyerman
通讯作者：
M. Shelden;S. Howitt;B. Kaiser;S. Tyerman
影响因子：
2.8
作者：
M. Zupin;A. Sedlar;M. Kidrič;V. Meglič
通讯作者：
M. Zupin;A. Sedlar;M. Kidrič;V. Meglič