Transportation container for pre-processing cytogenetic assays in radiation accidents.

Transportation container for pre-processing cytogenetic assays in radiation accidents.
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运输容器,用于预处理辐射事故中的细胞遗传学测定。

DOI:
10.1038/s41598-021-89832-x
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发表时间:
2021-05-17
期刊:
影响因子:
4.6
通讯作者:
Zenhausern F
Zenhausern F
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Gu J;Duane B;Repin M;Brenner DJ;Zenhausern F

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我们报告了一种运输集装箱,它通过在运输过程中预处理细胞培养物,为辐射对策的细胞遗传学生物剂量测定提供了颠覆性的物流。容器温度控制精确(<0.01°C),样品温度均匀(<0.1°C),满足生物剂量测定要求。使用现有的绝缘运输箱和长保质期的碱性电池使其成为国家库存的理想选择。使用集装箱进行的细胞遗传学生物剂量测定的剂量曲线显示出清晰的剂量反应,并且与使用实验室培养箱的对照剂量曲线具有高度线性相关性(皮尔逊相关系数:0.992)。该容器在运输过程中预处理生物样本的能力可能会对辐射对策产生重大影响,并对生物样本库、新型分子或细胞分析或疗法等其他应用产生潜在影响。
We report a shipping container that enables a disruptive logistics for cytogenetic biodosimetry for radiation countermeasures through pre-processing cell culture during transportation. The container showed precise temperature control (< 0.01 °C) with uniform sample temperature (< 0.1 °C) to meet the biodosimetry assay requirements. Using an existing insulated shipping box and long shelf life alkaline batteries makes it ideal for national stockpile. Dose curve of cytogenetic biodosimetry assay using the shipping container showed clear dose response and high linear correlation with the control dose curve using a laboratory incubator (Pearson’s correlation coefficient: 0.992). The container’s ability of pre-processing biological samples during transportation could have a significant impact on radiation countermeasure, as well as potential impacts in other applications such as biobanking, novel molecular or cell-based assays or therapies.
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