Formation of pea protein amyloid fibrils to stabilize high internal phase emulsions for encapsulation of lutein
Formation of pea protein amyloid fibrils to stabilize high internal phase emulsions for encapsulation of lutein
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形成豌豆蛋白淀粉样原纤维以稳定高内相乳液以封装叶黄素
DOI：
10.1016/j.jff.2022.105110
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发表时间：
2022-07
影响因子：
5.6
通讯作者：
Bing Hu
中科院分区：
文献类型：
--
作者：
Huaqian Wu;Yingqun Nian;Yanhua Liu;Yeyang Zhang;Bing Hu
·豌豆蛋白通过水解和重组形成具有多态性的原纤维。·豌豆蛋白纤维稳定HIPE，内相体积分数高达90%。·随着纤维含量的增加和混合分散速度的增加，HIPE增加。·出现了典型的高度堆积的多面体几何油滴微观结构。·HIPE包封叶黄素，提高了叶黄素对紫外线、热和Fe 3+的稳定性。在酸性环境中热处理豌豆纯化蛋白可诱导蛋白质水解和重组形成淀粉样纤维。豌豆淀粉样蛋白纤维具有高度的多态性，成熟纤维和蠕虫样纤维共存。豌豆淀粉样蛋白纤维可以稳定高内相乳液，内相体积分数高达90%。随着豌豆淀粉样蛋白原纤维含量的增加和分散速度的加快，HIPES的凝胶强度增大。在激光扫描共聚焦显微镜（CLSM）和低温扫描电子显微镜（Cryo-SEM）图像中均观察到了典型的多面几何油滴结构。叶黄素的负载量可达0.2wt%，且叶黄素的紫外稳定性、热稳定性和铁稳定性均得到显著提高。
• Pea protein formed fibrils with polymorphism via hydrolysis and re-assembly. • Pea protein fibrils stabilized HIPEs with internal phase volume fraction upto 90%. • HIPEs was enhanced with increase of fibril content and mixing dispersion speed. • Typical microstructure of highly packed polyhedral geometric oil droplets appeared. • The HIPEs encapsulated lutein and improved its stability against UV, heat and Fe 3+ . Thermal treatment of purified pea protein in acidic environment was found to induce the hydrolysis and re-assembly of the protein to form amyloid fibrils. Pea protein amyloid fibrils showed high polymorphism with the coexistence of mature and worm-like fibrils. Pea protein amyloid fibrils can stabilize the high internal phase emulsions (HIPEs) with the internal phase volume fraction as high as 90%. The gel strength of HIPEs was enhanced with the increase of pea protein amyloid fibril contents and the dispersion speed. The typical microstructure of highly packed polyhedral geometric oil droplets appeared in both the laser scanning confocal microscopy (CLSM) and Cryo-scan electron microscopy (Cryo-SEM) images of the HIPEs samples. High loading content of lutein upto 0.2 wt% in the prepared HIPEs was realized and the stability of lutein against ultraviolet irradiation, heat and iron was promoted significantly.
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影响因子：
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作者：
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影响因子：
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通讯作者：
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DOI：
10.1021/acssuschemeng.7b04868
发表时间：
2018-03
期刊：
ACS Sustainable Chem. Eng
影响因子：
--
作者：
Hongshan Zhu;Xiaoli Tan;Liqiang Tan;Huifang Zhang;Haining Liu;Tasawar Hayat;Xiangke Wang
通讯作者：
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DOI：
10.1021/acs.langmuir.7b04047
发表时间：
2018-04
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
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作者：
Huan Tan;Zhao Tu;H. Jia;X. Gou;To Ngai
通讯作者：
Huan Tan;Zhao Tu;H. Jia;X. Gou;To Ngai