Characterization of Four Novel Bacteriophages Isolated from British Columbia for Control of Non-typhoidal Salmonella in Vitro and on Sprouting Alfalfa Seeds.
Characterization of Four Novel Bacteriophages Isolated from British Columbia for Control of Non-typhoidal Salmonella in Vitro and on Sprouting Alfalfa Seeds.
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DOI：
10.3389/fmicb.2017.02193
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发表时间：
2017
影响因子：
5.2
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Fong K;LaBossiere B;Switt AIM;Delaquis P;Goodridge L;Levesque RC;Danyluk MD;Wang S
近年来，豆芽与北美许多沙门氏菌的爆发有关。通常，涉及氯、热和辐射的处理用于苜蓿种子消毒。然而，这些处理在其病原体控制的功效方面可能是高度可变的和/或对芽质量有害，因此被提倡天然替代品的消费者负面地感知。以前已经探索了使用噬菌体控制芽中的病原体，尽管结果相互矛盾和不一致。裂解酶是细菌的病毒捕食者，代表了一种有吸引力的方法，因为与常规治疗相比，它们提供了几个优点，例如它们对细菌靶标的高特异性及其在自然界中的普遍性。在这项研究中，从加拿大不列颠哥伦比亚省分离出四种沙门氏菌，并从宿主范围、爆发大小、潜伏期和环境稳定性方面进行了表征，以评估其控制沙门氏菌的潜力。噬菌体分离物SI 1表现出最大的宿主范围，最大的爆发大小和最短的潜伏期，在所有pH和温度下最大的稳定性，并且在控制S.体外肠溶试验。因此，选择SI 1作为人工污染苜蓿发芽种子的处理剂。感染复数（MOI）为110 PFU/CFU的肠炎。在噬菌体处理2小时后，观察到活沙门氏菌细胞显著（p < 0.05）减少38.3 ± 3.0%。在发芽过程的第二至第六天，也观察到沙门氏菌的减少，但与对照相比不显著（p > 0.05）。进一步证明噬菌体处理对芽产量影响不显著（p > 0.05）。这些结果强调了从不列颠哥伦比亚省环境中回收的沙门氏菌作为沙门氏菌生物防治剂的潜力，尽管观察到了不同的体外功效。此外，证明了SI 1在处理第1天显著（p < 0.05）控制发芽苜蓿种子上的沙门氏菌的有效性。虽然有希望，未来的工作应该旨在优化这种治疗，以实现更有效，更持久，在发芽苜蓿种子沙门氏菌的生物控制。
Alfalfa sprouts have been linked to numerous North American outbreaks of Salmonella in recent years. Conventionally, treatments involving chlorine, heat, and irradiation are used for alfalfa seed sanitation. However, such treatments may be highly variable in their efficacy for pathogen control and/or detrimental to sprout quality, therefore negatively perceived by consumers advocating for natural alternatives. The usage of bacteriophages for pathogen control in sprouts has been previously explored, although with conflicting and inconsistent results. Lytic phages, viral predators of bacteria, represent an attractive approach as they provide several advantages compared to conventional treatments, such as their high specificity for bacterial targets and their ubiquity in nature. In this study, four Salmonella phages were isolated from British Columbia, Canada and characterized with respect to host range, burst size, latent period, and environmental stability to assess their potential to control Salmonella. Phage isolate SI1 showed the greatest host range, highest burst size and shortest latent period, greatest stability across all pH and temperatures and was the most effective in control of S. Enteritidis in vitro. Therefore, SI1 was chosen for treatment of sprouting alfalfa seeds artificially contaminated with S. Enteritidis with a multiplicity of infection (MOI) of ∼110 PFU/CFU. A significant (p < 0.05) reduction of 38.3 ± 3.0% of viable Salmonella cells was observed following two h of phage treatment. On days two to six of the sprouting process, reductions of Salmonella were also observed, but were not significant compared to the control (p > 0.05). It was further demonstrated that the sprout yield was not significantly (p > 0.05) affected by phage treatment. These results highlight the potential of phages recovered from the British Columbia environment for use as biocontrol agents against Salmonella, although differing efficacies in vitro was observed. Moreover, the effectiveness of SI1 to significantly (p < 0.05) control Salmonella on sprouting alfalfa seeds on day 1 of treatment was demonstrated. Although promising, future work should aim to optimize this treatment to achieve more effective, and longer lasting, biocontrol of Salmonella in sprouting alfalfa seeds.
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影响因子：
2
作者：
Bari, M. L.;Enomoto, K.;Kawamoto, S.
通讯作者：
Kawamoto, S.
影响因子：
5.5
作者：
Jepson, CD;March, JB
通讯作者：
March, JB
影响因子：
4.6
作者：
Hamdi S;Rousseau GM;Labrie SJ;Tremblay DM;Kourda RS;Ben Slama K;Moineau S
通讯作者：
Moineau S
影响因子：
4.4
作者：
Goode, D;Allen, VM;Barrow, PA
通讯作者：
Barrow, PA
影响因子：
3.1
作者：
Kong, Qingke;Yang, Jiseon;Curtiss, Roy, III
通讯作者：
Curtiss, Roy, III