Redox regulation of glutathione S-transferase induction by benzyl isothiocyanate: correlation of enzyme induction with the formation of reactive oxygen intermediates.
Redox regulation of glutathione S-transferase induction by benzyl isothiocyanate: correlation of enzyme induction with the formation of reactive oxygen intermediates.
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异硫氰酸苄酯诱导谷胱甘肽 S-转移酶的氧化还原调节：酶诱导与活性氧中间体形成的相关性。
作者：
Y. Nakamura;H. Ohigashi;Seiji Masuda;Akira Murakami;Y. Morimitsu;Yoshiyuki Kawamoto;Toshihiko Osawa;Masayoshi Imagawa;Koji Uchida
在这里，我们报告了用化学预防癌症的异硫氰酸酯化合物苄基异硫氰酸酯（BITC）处理大鼠肝上皮RL34细胞诱导谷胱甘肽s -转移酶（GST）的分子机制。BITC可显著诱导RL34细胞的GST活性。Northern和Western blot分析表明，BITC特异性地促进了pi类GST同工酶（GSTP1）的产生。我们的研究首次证明，向细胞中添加BITC可立即增加荧光探针2',7'-二氯荧光素双醋酸酯检测到的活性氧中间体（roi）。BITC（10微米）处理细胞的roi水平比对照细胞高约50倍。此外，用马来酸二乙酯消耗谷胱甘肽显著增强bitc诱导的ROI产生，加速bitc诱导的GST活性升高，而用谷胱甘肽预处理细胞抑制ROI产生和GST诱导。异硫氰酸酯的构效关系也表明其产生roi的活性与其诱导gst的能力密切相关。此外，GSTP1增强子I-containing区域被发现是细胞内ROI诱导剂（如BITC和马来酸二乙酯）诱导GSTP1基因所必需的。这些数据表明，BITC参与了GSTP1诱导的氧化还原调控。
Here we report the molecular mechanism underlying the induction of glutathione S-transferase (GST) in rat liver epithelial RL34 cells treated with a cancer chemopreventive isothiocyanate compound, benzylisothiocyanate (BITC). BITC was found to significantly induce GST activity in RL34 cells. Northern and Western blot analyses demonstrated that BITC specifically enhanced the production of the class pi GST isozyme (GSTP1). Our studies demonstrated for the first time that the addition of BITC to the cells resulted in an immediate increase in the reactive oxygen intermediates (ROIs) detected by a fluorescence probe, 2',7'-dichlorofluorescin diacetate. The level of the ROIs in the cells treated with BITC (10 microM) was approximately 50-fold higher than those in the control cells. Furthermore, glutathione depletion by diethyl maleate significantly enhanced BITC-induced ROI production and accelerated the BITC-induced elevation of the GST activity, whereas pretreatment of the cells with glutathione inhibited both the ROI production and GST induction. The structure-activity relationship of the isothiocyanates also indicated that the ROI-producing activities closely correlated with their GST-inducing potencies. Moreover, the GSTP1 enhancer I-containing region was found to be essential for induction of the GSTP1 gene by intracellular ROI inducers such as BITC and diethyl maleate. These data suggest the involvement of the redox regulation on the induction of GSTP1 by BITC.
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醌诱导的氧化应激会升高大鼠肺上皮 L2 细胞中的谷胱甘肽并诱导 γ-谷氨酰半胱氨酸合成酶活性。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Shi,MM;Kugelman,A;Iwamoto,T;Tian,L;Forman,HJ
通讯作者：
Forman,HJ
影响因子：
4.4
作者：
D. Bass;J. W. Parce;L. Dechatelet;P. Szejda;M. Seeds;M. Thomas
通讯作者：
D. Bass;J. W. Parce;L. Dechatelet;P. Szejda;M. Seeds;M. Thomas
DOI：
10.1006/bbrc.1998.9729
发表时间：
1998-12-18
影响因子：
3.1
作者：
Li, YB;Trush, MA
通讯作者：
Trush, MA
通过流式细胞术分析检测源自鼠纤维肉瘤或人肾细胞癌的细胞中细胞谷胱甘肽的异质性。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Shrieve,DC;Bump,EA;Rice,GC
通讯作者：
Rice,GC
影响因子：
2.9
作者：
CATHCART, R;SCHWIERS, E;AMES, BN
通讯作者：
AMES, BN