Molecular investigations on the prevalence and viral load of enteric viruses in pigs from five European countries.
Molecular investigations on the prevalence and viral load of enteric viruses in pigs from five European countries.
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DOI：
10.1016/j.vetmic.2015.10.019
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发表时间：
2016
影响因子：
3.3
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Zhou W;Ullman K;Chowdry V;Reining M;Benyeda Z;Baule C;Juremalm M;Wallgren P;Schwarz L;Zhou E;Pedrero SP;Hennig-Pauka I;Segales J;Liu L
猪星状病毒4型在病毒载量较高的49个欧洲养殖场中流行。轮状病毒主要在腹泻猪身上发现。柯布病毒和猪圆环病毒2普遍存在。猪的肠道病毒感染可能导致腹泻，导致节俭和重大经济损失。本研究报告了来自欧洲五个国家(奥地利、德国、匈牙利、西班牙和瑞典)49个养殖场的419头猪的肠道感染情况，其中包括猪星状病毒4型(PAstV4)、猪A组轮状病毒(GARV)、猪C组轮状病毒(GCRV)、猪圆环病毒2型(PCV2)和猪柯布病毒(Pkov)。建立了实时RT-PCR分析来检测粪便样本，并比较患病率和病毒载量与健康状况、来源农场和年龄组的关系。结果表明，病毒种类以PAstV4为主(70.4%)，其次为pKOV(56.7%)、PCV2(42.2%)、GCRV(3%)和GARV(0.9%)。苗圃组和生长育肥组腹泻型猪的PAstV4病毒载量较高。轮状病毒主要在腹泻猪中检测到，而PCV2在临床健康猪中检测到的频率高于腹泻猪，这表明大多数PCV2感染是亚临床感染。PAstV4、PCV2和pKOV被认为在欧洲猪畜中普遍存在，它们之间的混合感染频繁，与疾病状态无关，与典型轮状病毒感染的低流行率形成对比。
Porcine astrovirus type 4 was prevalent in the 49 European farms with a high viral load. Rotaviruses were mainly found in diarrheic pigs. Kobuvirus and porcine circovirus 2 were ubiquitous. Enteric viral infections in pigs may cause diarrhea resulting in ill-thrift and substantial economic losses. This study reports the enteric infections with porcine astrovirus type 4 (PAstV4), porcine group A rotavirus (GARV), porcine group C rotavirus (GCRV), porcine circovirus type 2 (PCV2) and porcine kobuvirus (PKoV) in 419 pigs, comprising both healthy and diarrheic animals, from 49 farms in five European countries (Austria, Germany, Hungary, Spain and Sweden). Real-time RT-PCR assays were developed to test fecal samples and to compare the prevalence and viral load in relation to health status, farms of origin and age groups. The results showed that PAstV4 (70.4%) was the dominant virus species, followed by PKoV (56.7%), PCV2 (42.2%), GCRV (3%) and GARV (0.9%). Diarrheic pigs had a higher viral load of PAstV4 in the nursery and growing-finishing groups. Rotaviruses were mainly detected in diarrheic pigs, whereas PCV2 was more often detected in clinically healthy than in diarrheic pigs, suggesting that most PCV2 infections were subclinical. PAstV4, PCV2 and PKoV were considered ubiquitous in the European pig livestock and co-infections among them were frequent, independently of the disease status, in contrast to a low prevalence of classical rotavirus infections.
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影响因子：
3.2
作者：
Brnic, Dragan;Prpic, Jelena;Jemersic, Lorena
通讯作者：
Jemersic, Lorena
影响因子：
3.3
作者：
Dupont, K.;Nielsen, E. O.;Larsen, L. E.
通讯作者：
Larsen, L. E.
影响因子：
3.3
作者：
Gagnon, Carl A.;Music, Nedzad;Harel, Josee
通讯作者：
Harel, Josee
影响因子：
2.4
作者：
Di Profio, Federica;Ceci, Chiara;Di Martino, Barbara
通讯作者：
Di Martino, Barbara
影响因子：
8.8
作者：
Liu, Jie;Kibiki, Gibson;Houpt, Eric
通讯作者：
Houpt, Eric