Study of Terpenoid Synthesis and Prenyltransferase in Roots of Rehmannia glutinosa Based on iTRAQ Quantitative Proteomics.
Study of Terpenoid Synthesis and Prenyltransferase in Roots of Rehmannia glutinosa Based on iTRAQ Quantitative Proteomics.
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基于iTRAQ定量蛋白质组学研究地黄根中萜类化合物合成及异戊烯基转移酶
DOI：
10.3389/fpls.2021.693758
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发表时间：
2021
影响因子：
5.6
通讯作者：
Duan H
中科院分区：
文献类型：
--
作者：
Chen P;Wei X;Qi Q;Jia W;Zhao M;Wang H;Zhou Y;Duan H
地黄具有重要的药用价值；萜类化合物是红花中的主要活性成分之一。本研究采用iTRAQ技术分析了R. glutinosa根中蛋白质的相对丰度，对6,752个可靠的蛋白质进行了定量。 GO富集结果表明，大部分蛋白质参与代谢过程或细胞过程，57.63%的蛋白质具有催化活性，65.80%的蛋白质富集在膜结合细胞器中。在R. glutinosa的根中，有38个有意义的KEGG富集，在一些途径中发现了更多的DEP，特别是蛋白酶体途径和TCA循环，在根的伸长阶段和扩展阶段之间有15.0％的DEP。此外，还发现了五种萜类化合物合成的KEGG途径。大多数异戊烯基转移酶属于 FPP/GGPP 合酶家族，参与萜类骨架生物合成，并且都与生物素羧化酶 CAC2 相互作用。与伸长期相比，许多异戊烯基转移酶在根的扩张期或成熟期表现出较高的表达量。此外，从R. glutinosa中克隆了8个FPP/GGPP合酶编码基因，分别为FPPS、FPPS1、GGPS、GGPS3、GGPS4、GGPS5、GPPS和GPPS2，在FPPS、FPPS1、GGPS5和GGPS2中也发现了内含子，且FPP/GPP合酶在生物体中较为保守，尤其是在绿色植物中，其中GPPS与GPPS共存。或 GPPS2 在植物中显着较高。进一步分析发现，地黄的FPP/GGPP合酶分为GGPS、GPPS和FPPS 3种，其基因表达在不同地黄的品种、生长期或组织中存在显着差异。与GGPS相比，GPPS和FPPS在R. glutinosa中的表达量明显较高，尤其是在膨大期和成熟期。因此，R. glutinosa根中萜类化合物的合成受到复杂的调控，需要进一步研究。
Rehmannia glutinosa has important medicinal value; terpenoid is one of the main active components in R. glutinosa. In this study, iTRAQ technique was used to analyze the relative abundance of proteins in roots of R. glutinosa, and 6,752 reliable proteins were quantified. GO enrichment results indicated that most proteins were involved in metabolic process or cellular process, 57.63% proteins had catalytic activity, and 65.80% proteins were enriched in membrane-bounded organelle. In roots of R. glutinosa, there were 38 KEGG enrichments with significance, more DEPs were found in some pathways, especially the proteasome pathway and TCA cycle with 15.0% DEPs between elongation stage and expansion stage of roots. Furthermore, five KEGG pathways of terpenoid synthesis were found. Most prenyltransferases belong to FPP/GGPP synthase family, involved in terpenoid backbone biosynthesis, and all interacted with biotin carboxylase CAC2. Compared with that at the elongation stage, many prenyltransferases exhibited higher expression at the expansion stage or maturation stage of roots. In addition, eight FPP/GGPP synthase encoding genes were cloned from R. glutinosa, namely FPPS, FPPS1, GGPS, GGPS3, GGPS4, GGPS5, GPPS and GPPS2, introns were also found in FPPS, FPPS1, GGPS5 and GGPS2, and FPP/GPP synthases were more conservative in organisms, especially in viridiplantae, in which the co-occurrence of GPPS or GPPS2 was significantly higher in plants. Further analysis found that FPP/GGPP synthases of R. glutinosa were divided into three kinds, GGPS, GPPS and FPPS, and their gene expression was significantly diverse in different varieties, growth periods, or tissues of R. glutinosa. Compared with that of GGPS, the expression of GPPS and FPPS was much higher in R. glutinosa, especially at the expansion stage and maturation stage. Thus, the synthesis of terpenoids in roots of R. glutinosa is intricately regulated and needs to be further studied.
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影响因子：
5.1
作者：
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通讯作者：
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影响因子：
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影响因子：
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通讯作者：
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