Multiplex PCR Assay for Clade Typing of Salmonella enterica Serovar Enteritidis.
Multiplex PCR Assay for Clade Typing of Salmonella enterica Serovar Enteritidis.
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DOI：
10.1128/spectrum.03182-22
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发表时间：
2022-12-21
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
肠道沙门氏菌（Salmonella enterica serovar Enteritime）是引起人类疾病的非伤寒沙门氏菌的最常见报告的血清型之一，并且在全球范围内引起胃肠炎和侵袭性非伤寒沙门氏菌（iNTS）疾病。来自世界各地的沙门氏菌分离株的全基因组序列（WGS）比较已经确定了三个不同的分支，全球流行，中非/东非和西非，所有这些都与流行病有关：全球流行分支与家禽相关的胃肠炎有关，而两个非洲分支与iNTS疾病有关。然而，这些分支在非洲的分布和流行病学知之甚少，因为这些分支的鉴定目前需要全基因组测序能力。在这里，我们报告了一个敏感的，时间和成本效益的实时PCR检测能够区分沙门氏菌肠杆菌分支，以促进监测和告知公共卫生反应。本文所述的试验仅限于先前确认的S。肠杆菌分离株。撒哈拉以南非洲地区侵袭性肠道沙门氏菌血流感染的诊断和治疗面临挑战，导致病死率约为15%。重要的是要确定不同的分支的S。在非洲环境中的诊断实验室进行肠道试验，以确定与特定疫情相关的不同健康结果。在这里，我们描述了一个高质量的分子分类检测的发展分支分型的S。肠内营养是理想的用于公共卫生实验室在资源有限的设置。
Salmonella enterica serovar Enteritidis is one of the most commonly reported serovars of nontyphoidal Salmonella causing human disease and is responsible for both gastroenteritis and invasive nontyphoidal Salmonella (iNTS) disease worldwide. Whole-genome sequence (WGS) comparison of Salmonella Enteritidis isolates from across the world has identified three distinct clades, global epidemic, Central/East African, and West African, all of which have been implicated in epidemics: the global epidemic clade was linked to poultry-associated gastroenteritis, while the two African clades were related to iNTS disease. However, the distribution and epidemiology of these clades across Africa are poorly understood because identification of these clades currently requires whole-genome sequencing capacity. Here, we report a sensitive, time- and cost-effective real-time PCR assay capable of differentiating between the Salmonella Enteritidis clades to facilitate surveillance and to inform public health responses. The assay described here is limited to previously confirmed S. Enteritidis isolates. IMPORTANCE Challenges in the diagnosis and treatment of invasive Salmonella Enteritidis bloodstream infections in sub-Saharan Africa are responsible for a case fatality rate of approximately 15%. It is important to identify distinct clades of S. Enteritidis in diagnostic laboratories in the African setting to determine the different health outcomes associated with particular outbreaks. Here, we describe the development of a high-quality molecular classification assay for clade typing of S. Enteritidis that is ideal for use in public health laboratories in resource-limited settings.
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影响因子：
9.4
作者：
Liebana, E;Garcia-Migura, L;Woodward, MJ
通讯作者：
Woodward, MJ
影响因子：
168.9
作者：
Feasey, Nicholas A.;Dougan, Gordon;Kingsley, Robert A.;Heyderman, Robert S.;Gordon, Melita A.
通讯作者：
Gordon, Melita A.
影响因子：
12.3
作者：
Perez-Sepulveda BM;Heavens D;Pulford CV;Predeus AV;Low R;Webster H;Dykes GF;Schudoma C;Rowe W;Lipscombe J;Watkins C;Kumwenda B;Shearer N;Costigan K;Baker KS;Feasey NA;Hinton JCD;Hall N;10KSG consortium
通讯作者：
10KSG consortium
影响因子：
3.9
作者：
Darboe, Saffiatou;Bradbury, Richard S.;Phelan, Jody;Kanteh, Abdoulie;Muhammad, Abdul-Khalie;Worwui, Archibald;Yang, Shangxin;Nwakanma, Davis;Perez-Sepulveda, Blanca;Kariuki, Samuel;Kwambana-Adams, Brenda;Antonio, Martin
通讯作者：
Antonio, Martin
影响因子：
56.3
作者：
Stanaway, Jeffrey D.;Parisi, Andrea;Crump, John A.
通讯作者：
Crump, John A.