Two New Lytic Bacteriophages of the Myoviridae Family Against Carbapenem-Resistant Acinetobacter baumannii.
Two New Lytic Bacteriophages of the Myoviridae Family Against Carbapenem-Resistant Acinetobacter baumannii.
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DOI：
10.3389/fmicb.2018.00850
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发表时间：
2018
影响因子：
5.2
通讯作者：
Zong Z
中科院分区：
文献类型：
--
作者：
Zhou W;Feng Y;Zong Z
从医院污水中分离到2株溶菌噬菌体WCHABP 1和WCHABP 12，分别感染18株碳青霉烯类耐药鲍曼不动杆菌中的9株和12株。电子显微镜扫描显示，这两种噬菌体具有与肌尾病毒科噬菌体相似的形态。全基因组测序结果显示，WCHABP 1和WCHABP 12的基因组大小分别为45.4和45.8 kb，GC含量为37.6%，与Myoviridae科Ap 22病毒属噬菌体的DNA序列具有显著的相似性。因此，根据国际病毒分类委员会批准的建议进行分类学分析，确认WCHABP 1和WCHABP 12代表Ap 22病毒属的两个新种。两株噬菌体均不含tRNA，但含有88个和89个开放阅读框（ORF），其中22个和21个具有已知功能，编码与形态发生、包装、裂解和核苷酸代谢有关的蛋白质。纤维尾蛋白的大单位的C-末端氨基酸在噬菌体之间变化，这可以解释它们不同的宿主范围。对于大多数裂解性噬菌体，裂解需要一组孔蛋白和内溶素。然而，在WCHABP 1和WCHABP 12中没有鉴定出已知的holin编码基因，这表明它们可能使用替代的、尚未鉴定的新型holin用于宿主细胞膜裂解。为了检测噬菌体对A.鲍曼不动杆菌感染时，使用大蜡螟幼虫模型。只有<20%的G。耐碳青霉烯类抗生素的大蜡螟幼虫感染后96 h存活。鲍曼不动杆菌菌株，从中回收了两种噬菌体。在施用WCHABP 1和WCHABP 12的情况下，幼虫的存活率增加到75%，而多粘菌素B的处理仅将存活率略微增加到25%。本研究中分离到的两种新的裂解性噬菌体可以扩大我们对不动杆菌噬菌体的认识，并可能为抗不动杆菌噬菌体提供新的潜在治疗选择。鲍曼不动杆菌。
Two lytic bacteriophages, WCHABP1 and WCHABP12, were recovered from hospital sewage and were able to infect 9 and 12 out of 18 carbapenem-resistant Acinetobacter baumannii clinical strains, which belonged to different clones. Electron microscopy scan showed that both bacteriophages had the similar morphology as those of the Myoviridae family. Whole genomic sequencing revealed 45.4- or 45.8-kb genome with a 37.6% GC content for WCHABP1 and WCHABP12, both of which showed significant DNA sequence similarity with bacteriophages of the Ap22virus genus within the Myoviridae family. Taxonomic analysis was therefore performed following the proposal approved by the International Committee on Taxonomy of Viruses, which confirmed that WCHABP1 and WCHABP12 represented two new species of the Ap22virus genus. No tRNAs but 88 and 89 open reading frames (ORFs) were predicted for the two bacteriophages, among which 22 and 21 had known function and encoded proteins for morphogenesis, packaging, lysis, and nucleiotide metabolism. The C-terminal amino acids of the large unit of fiber tail proteins varied between the bacteriophages, which may explain their different host ranges. For most lytic bacteriophages, a set of holin and endolysin are required for lysis. However, no known holin-encoding genes were identified in WCHABP1 and WCHABP12, suggesting that they may use alternative, yet-to-be-identified, novel holins for host cell membrane lysis. To test the efficacy of the bacteriophages in protecting against A. baumannii infection, a Galleria mellonella larva model was used. Only <20% G. mellonella larvae survived at 96 h after being infected by carbapenem-resistant A. baumannii strains, from which the two bacteriophages were recovered. With the administration of WCHABP1 and WCHABP12, the survival of larvae increased to 75%, while the treatment of polymyxin B only slightly increased the survival rate to 25%. The isolation of two new lytic bacteriophages in this study could expand our sight on Acinetobacter bacteriophages and may offer new potential therapeutic alternatives against A. baumannii.
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影响因子：
3.7
作者：
Bryan MJ;Burroughs NJ;Spence EM;Clokie MR;Mann NH;Bryan SJ
通讯作者：
Bryan SJ
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
6.5
作者：
Anisimova, Maria;Gascuel, Olivier
通讯作者：
Gascuel, Olivier
DOI：
10.1016/j.ijantimicag.2012.11.013
发表时间：
2013-03-01
影响因子：
10.8
作者：
Hornsey, Michael;Phee, Lynette;Wareham, David W.
通讯作者：
Wareham, David W.
DOI：
10.1007/978-1-60327-565-1_7
发表时间：
2009
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Casjens, Sherwood R;Gilcrease, Eddie B
通讯作者：
Gilcrease, Eddie B