Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness.
Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness.
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背膘厚度极端表型沂蒙黑猪肝脏 mRNA 和 miRNA 表达谱综合分析
DOI：
10.18632/oncotarget.21918
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发表时间：
2017-12-29
期刊：
影响因子：
--
通讯作者：
Li K
中科院分区：
文献类型：
--
作者：
Li W;Yang Y;Liu Y;Liu S;Li X;Wang Y;Zhang Y;Tang H;Zhou R;Li K
脂肪沉积是家畜的一项重要经济性状，也是人类肥胖相关疾病的重要组成部分，而肝脏是调节哺乳动物脂肪合成和代谢的中枢器官。本研究利用RNA-Seq和miRNA-Seq技术对背脂厚度存在差异的两组猪(H组和L组)的肝脏转录组进行了分析，以进一步探讨脂肪沉积的分子机制。在H组和L组之间共鉴定出238个差异表达基因(DIG)和58个差异表达miRNAs。这些基因和miRNAs在功能上与脂质代谢相关，包括CYP1A1/2、HMGCS2、ACSS2、UBE2L6、miR-27A和miR-31。功能浓缩分析表明，与氧化应激相关的基因可能与猪的脂肪沉积有关。鉴定了两个参与脂肪代谢的miRNA-mRNA相互作用网络，这为决定这些猪脂肪含量的分子调控提供了新的见解。总体而言，我们的研究进一步加深了我们对脂肪沉积所涉及的分子机制的理解，这些结果可能有助于设计选择策略，以提高猪肉的质量，并与人类肥胖作斗争。
Fat deposition is an important economic trait in farm animals as well as obesity related diseases in humans, and the liver is a central organ involved in regulating lipid synthesis and metabolism in mammals. In this study, the pig liver transcriptome of two groups (H and L) showing differences in backfat thickness were profiled using RNA-Seq and miRNA-Seq to further explore the molecular mechanism of fat deposition. A total of 238 differentially expressed genes (DEGs) and 58 differentially expressed miRNAs were identified between the H and L group. These genes and miRNAs were functionally related to lipid metabolism, including CYP1A1/2, HMGCS2, ACSS2, UBE2L6, miR-27a, and miR-31. Functional enrichment analysis revealed that genes associated with oxidative stress might be responsible for fat deposition in pigs. Two miRNA-mRNA interaction networks involved in lipid metabolism were identified, and these provided new insights into the molecular regulation that determines fat content in these pigs. Overall, our study furthers our understanding of the molecular mechanisms involved in fat deposition, and these results may help in the design of selection strategies to improve the quality of pork meat and to combat obesity in humans.
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影响因子：
5.8
作者：
Alexander R;Lodish H;Sun L
通讯作者：
Sun L
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1152/ajpregu.00049.2014
发表时间：
2014-07-01
期刊：
American journal of physiology. Regulatory, integrative and comparative physiology
影响因子：
--
作者：
Alfaradhi MZ;Fernandez-Twinn DS;Martin-Gronert MS;Musial B;Fowden A;Ozanne SE
通讯作者：
Ozanne SE
影响因子：
64.8
作者：
通讯作者：
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影响因子：
5.3
作者：
Fu, Ting;Seok, Sunmi;Kemper, Jongsook Kim
通讯作者：
Kemper, Jongsook Kim