Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila.
Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila.
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嗜水气单胞菌感染的团头鲂 (Megalobrama amlycephala) 肝脏中 MicroRNA 的鉴定和表征
DOI：
10.3390/ijms17121972
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发表时间：
2016-11-25
影响因子：
5.6
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Cui L;Hu H;Wei W;Wang W;Liu H
MicroRNA（miRNAs）是一类在多种生物过程调控中发挥关键作用的小分子RNA。为了更好地理解在团头鲂（Megalobrama amblycephala）中的嗜水气单胞菌（Aeromonaserina）感染的背景下miRNA的生物学意义，使用Illumina深度测序技术对在感染后0（非感染）、4和24 h（poi）从鱼肝脏获得的小RNA文库进行测序。从这三个RNA文库分别获得总共11，244，207、9，212，958和7，939，157个干净读段。生物信息学分析鉴定出171个保守的miRNAs和62个新的miRNAs。通过RT-PCR验证了10个随机选择的新miRNAs的存在。成对比较表明，分别有61和44个miRNAs在4和24 h poi时差异表达。此外，通过qRT-PCR验证9个随机选择的miRNA的表达谱。MicroRNA靶标预测、基因本体（GO）注释和京都基因与基因组百科全书（KEGG）分析表明，多种生物学途径可能受到A.尿道感染。此外，转铁蛋白（TF）和转铁蛋白受体（TFR）基因被证实是miR-375的直接靶点。这些结果将扩大我们对miRNAs在M. amblycephala到A.的感染，并促进制定有效的战略，以防止A。M.钝头虫。
MicroRNAs (miRNAs) are small RNA molecules that play key roles in regulation of various biological processes. In order to better understand the biological significance of miRNAs in the context of Aeromonas hydrophila infection in Megalobrama amblycephala, small RNA libraries obtained from fish liver at 0 (non-infection), 4, and 24 h post infection (poi) were sequenced using Illumina deep sequencing technology. A total of 11,244,207, 9,212,958, and 7,939,157 clean reads were obtained from these three RNA libraries, respectively. Bioinformatics analysis identified 171 conserved miRNAs and 62 putative novel miRNAs. The existence of ten randomly selected novel miRNAs was validated by RT-PCR. Pairwise comparison suggested that 61 and 44 miRNAs were differentially expressed at 4 and 24 h poi, respectively. Furthermore, the expression profiles of nine randomly selected miRNAs were validated by qRT-PCR. MicroRNA target prediction, gene ontology (GO) annotation, and Kyoto Encylopedia of Genes and Genomes (KEGG) analysis indicated that a variety of biological pathways could be affected by A. hydrophila infection. Additionally, transferrin (TF) and transferrin receptor (TFR) genes were confirmed to be direct targets of miR-375. These results will expand our knowledge of the role of miRNAs in the immune response of M. amblycephala to A. hydrophila infection, and facilitate the development of effective strategies against A. hydrophila infection in M. amblycephala.
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