AtMYB14 Regulates Cold Tolerance in Arabidopsis.

AtMYB14 Regulates Cold Tolerance in Arabidopsis.
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DOI:
10.1007/s11105-012-0481-z
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发表时间:
2013
影响因子:
2.1
通讯作者:
Qin, Genji
Qin, Genji
中科院分区:
生物学4区
文献类型:
--
作者:
Chen, Yan;Chen, Zhangliang;Kang, Juqing;Kang, Dingming;Gu, Hongya;Qin, Genji

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低温影响植物生长和作物产量。CBF基因是一类在冷反应中起重要作用的转录因子。在这里,我们报告AtMYB 14通过影响CBF基因的表达参与拟南芥的抗冻性。AtMYB 14基因在低温处理下表达下调。AtMYB 14编码作为R2 R3-MYB转录激活因子的核蛋白。通过人工microRNA敲低AtMYB 14基因可提高植物对冷冻胁迫的耐受性。在低温处理下,AtMYB 14基因敲除植株中CBF基因及其下游基因的表达水平均显著高于野生型。我们的研究结果表明AtMYB 14在植物对冷胁迫的反应中起着重要的作用。
Low temperature affects plant growth and crop productivity. The CBF genes are a class of transcription factors that play important roles in cold response. Here we report that AtMYB14 participates in freezing tolerance in Arabidopsis by affecting expression of CBF genes. The AtMYB14 gene was down-regulated by cold treatment. AtMYB14 encodes a nuclear protein that functions as an R2R3-MYB transcription activator. Knock-down of AtMYB14 by artificial microRNA increased the tolerance to freezing stress. Both the CBF genes and the downstream genes were induced to a much higher level in AtMYB14 knock-down plants than in wild type under cold treatment. Our results suggest that AtMYB14 plays an important role in the plant response to cold stress.
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