Orthotopic transplantation of neonatal GFP rat ovary as experimental model to study ovarian development and toxicology.
Orthotopic transplantation of neonatal GFP rat ovary as experimental model to study ovarian development and toxicology.
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DOI：
10.1016/j.reprotox.2008.09.001
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发表时间：
2008-11
影响因子：
3.3
通讯作者：
Uzumcu, Mehmet
中科院分区：
文献类型：
--
作者：
Marano, Jason E.;Sun, Dongming;Zama, Aparna Mahakali;Young, Wise;Uzumcu, Mehmet
大鼠是生物医学研究中最常用的实验动物之一。因此，在老鼠身上的新研究工具的可用性可以在这种哺乳动物广泛使用的领域提供相当大的进步。我们报道了一种新的适合器官移植的绿色荧光蛋白（GFP）大鼠菌株的发展，以及将新生卵巢原位移植到野生型Fischer 344 （F344）菌株后GFP大鼠的出生。将eGFP Sprague-Dawley （SD-Tg(CAG-EGFP)Cz-004Osb）与野生型F344回交8代，得到一株新的GFP大鼠。将出生后8只GFP大鼠的全卵巢原位移植至双侧卵巢切除的野生型成年雌性（n = 6）。所有的接受者都进行了交配，结果5窝中有3窝含有GFP幼崽。在PND 8组中，所有受者周期正常，移植后9个月采集卵巢形态正常。在PND 21组中，60%的受者在移植后9个月表现出正常的发情周期，但卵巢大小缩小。这种新菌株和新生儿原位移植可用于许多生物医学领域，包括移植、发育和生殖毒理学。
The rat is one of the most commonly used experimental animal species in biomedical research. The availability of new research tools in rats could therefore provide considerable advances in the areas where this mammal is extensively used. We report the development of a new Green Fluorescent Protein (GFP) rat strain suitable for organ transplantation and the birth of GFP rats following orthotopic transplantation of neonatal ovaries from this newly developed GFP rat strain to a wild-type Fischer 344 (F344) strain. A new GFP rat strain was developed by backcrossing eGFP Sprague-Dawley (SD-Tg(CAG-EGFP)Cz-004Osb) to wild-type F344 for eight generations. Whole ovaries from postnatal day 8 GFP rats were transplanted orthotopically to bilaterally ovariectomized wild-type adult females (n = 6). All recipients were mated, and three of the five resulting litters contained GFP pups. In the PND 8 group, all recipients cycled regularly and the ovarian morphology appeared normal when collected at 9 months post-transplantation. In the PND 21 group, 60% of the recipients displayed regular estrous cycles at 9 months post-transplantation, but showed reduced ovarian size. This new strain and neonatal orthotopic transplantation could be useful for many biomedical fields including transplantation, development, and reproductive toxicology.
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影响因子：
4.8
作者：
OJEDA, SR;RAMIREZ, VD
通讯作者：
RAMIREZ, VD
影响因子：
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作者：
Kezele, P;Skinner, MK
通讯作者：
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通讯作者：
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通讯作者：
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