Proteomic Analysis Reveals That Metabolic Flows Affect the Susceptibility of Aeromonas hydrophila to Antibiotics.
Proteomic Analysis Reveals That Metabolic Flows Affect the Susceptibility of Aeromonas hydrophila to Antibiotics.
复制标题
蛋白质组学分析揭示代谢流影响嗜水气单胞菌对抗生素的敏感性
DOI：
10.1038/srep39413
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发表时间：
2016-12-19
影响因子：
4.6
通讯作者：
Lin X
中科院分区：
文献类型：
--
作者：
Yao Z;Li W;Lin Y;Wu Q;Yu F;Lin W;Lin X
抗生素的过度使用导致抗生素耐药性的发展，并限制了这些药物在对抗细菌方面的使用寿命，包括气单胞菌，这是一种众所周知的机会致病菌，会导致鱼类和其他动物的严重感染。本研究对A.通过多种蛋白质组学方法对Escherichia。采用二甲基标记法和非标记法比较了不同剂量土霉素（OXY）对蛋白质表达的影响。结果表明，在响应OXY应激时，翻译过程增加了这些蛋白质的丰度，而主要是中心代谢途径降低了它们的丰度。为了证实我们的假设，将各种外源性代谢物与OXY复合，并测量所产生的存活能力。结果表明，苹果酸、丝氨酸、蛋氨酸等7种代谢产物在大肠杆菌中均有表达。显著降低了A.在OXY存在下，Escherichia，而4种代谢物（精氨酸，赖氨酸，酪氨酸等）做了相反的事进一步的研究表明，包含外源性代谢物的化合物与各种抗生素的组合可以具有显著的杀菌效果，并且可以广泛使用，特别是与四环素抗生素一起使用。这些发现可能为A.尿道感染。
The overuse of antibiotics results in the development of antibiotic resistance and limits the useful life of these drugs in fighting bacteria, including Aeromonas hydrophila, a well-known opportunistic pathogen that causes serious infections in fish and other animals. In this study, we investigated the adaptive resistance mechanism in A. hydrophila by multiple proteomic methods. Dimethyl labeling and label-free methods were performed to compare the differential expression of proteins in response to various doses of oxytetracycline (OXY). The results point to the conclusions that, in response to OXY stress, translational processes increase the abundance of these proteins whereas largely central metabolic pathways decrease their abundance. To confirm our hypothesis, various exogenous metabolites were compounded with OXY, and the resulting survival capabilities were measured. Results show that 7 metabolites (malic acid, serine, methionine, etc.) significantly decreased the survival capabilities of A. hydrophila in the presence of OXY, whereas 4 metabolites (arginine, lysine, tyrosine, etc.) did the opposite. Further investigation suggests that a compound comprising exogenous metabolites in combination with various antibiotics could have a significant bactericidal effect and might come into widespread use, especially together with tetracycline antibiotics. These findings may provide new clues to the antimicrobial treatment of A. hydrophila infection.
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