Discovery of a Novel Periodontal Disease-Associated Bacterium.
Discovery of a Novel Periodontal Disease-Associated Bacterium.
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DOI：
10.1007/s00248-018-1200-6
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发表时间：
2019-01
影响因子：
3.6
通讯作者：
Edlund A
中科院分区：
文献类型：
--
作者：
Torres PJ;Thompson J;McLean JS;Kelley ST;Edlund A
牙周炎（PD）是世界上最常见的传染病之一，其来源于以生物膜形式生长的口腔细菌群落。牙菌斑）在牙周袋中的牙齿和牙龈表面上。与牙周病相关的细菌会引发免疫细胞的炎症反应，在疾病的后期阶段会导致支持牙齿的软组织和硬组织结构的丧失。到目前为止，只有少数细菌被鉴定为PD的感染因子。尽管深度测序技术，如全群落鸟枪测序，有可能在任何给定的环境中捕获高度复杂的细菌群落的详细信息，但我们仍然缺乏与PD和其他疾病相关的口腔微生物组的主要参考基因组。在最近的工作中，通过使用监督机器学习和基因组组装的组合，我们从牙周样本中的拟杆菌门的一个新成员中识别出了一个基因组。在这里，通过应用比较宏基因组学读取分类方法，包括来自不同人体部位的272个宏基因组，以及我们先前组装的未培养的棘突拟杆菌（CBP）细菌的基因组草案，我们显示CBP在牙菌斑中无处不在的分布，以及其与驻留在深层牙周袋中的众所周知的致病性“红色复合物”的强烈关联。本文的在线版本（10.1007/s 00248 -018-1200-6）包含补充材料，可供授权用户使用。
One of the world’s most common infectious disease, periodontitis (PD), derives from largely uncharacterized communities of oral bacteria growing as biofilms (a.k.a. plaque) on teeth and gum surfaces in periodontal pockets. Bacteria associated with periodontal disease trigger inflammatory responses in immune cells, which in later stages of the disease cause loss of both soft and hard tissue structures supporting teeth. Thus far, only a handful of bacteria have been characterized as infectious agents of PD. Although deep sequencing technologies, such as whole community shotgun sequencing have the potential to capture a detailed picture of highly complex bacterial communities in any given environment, we still lack major reference genomes for the oral microbiome associated with PD and other diseases. In recent work, by using a combination of supervised machine learning and genome assembly, we identified a genome from a novel member of the Bacteroidetes phylum in periodontal samples. Here, by applying a comparative metagenomics read-classification approach, including 272 metagenomes from various human body sites, and our previously assembled draft genome of the uncultivated Candidatus Bacteroides periocalifornicus (CBP) bacterium, we show CBP’s ubiquitous distribution in dental plaque, as well as its strong association with the well-known pathogenic “red complex” that resides in deep periodontal pockets. The online version of this article (10.1007/s00248-018-1200-6) contains supplementary material, which is available to authorized users.
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影响因子：
11
作者：
Duran-Pinedo, Ana E.;Chen, Tsute;Frias-Lopez, Jorge
通讯作者：
Frias-Lopez, Jorge
过氧化还原蛋白 1 (Prx1) 是一种双功能酶，具有不依赖半胱氨酸的过氧化氢酶样活性
DOI：
10.1042/bcj20160851
发表时间：
2017-04-04
期刊：
The Biochemical journal
影响因子：
--
作者：
Sun CC;Dong WR;Shao T;Li JY;Zhao J;Nie L;Xiang LX;Zhu G;Shao JZ
通讯作者：
Shao JZ
影响因子：
3.7
作者：
Bushnell B;Rood J;Singer E
通讯作者：
Singer E
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
3.7
作者：
Hajishengallis G;Lamont RJ
通讯作者：
Lamont RJ