Two-photon imaging of calcium in virally transfected striate cortical neurons of behaving monkey.
Two-photon imaging of calcium in virally transfected striate cortical neurons of behaving monkey.
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DOI：
10.1371/journal.pone.0013829
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发表时间：
2010-11-04
期刊：
影响因子：
3.7
通讯作者：
Siegel RM
中科院分区：
文献类型：
--
作者：
Heider B;Nathanson JL;Isacoff EY;Callaway EM;Siegel RM
双光子扫描显微镜提高了我们对非哺乳动物和哺乳动物神经信号的理解。需要在非人类灵长类动物执行行为任务的长时间内执行双光子扫描显微镜。在两只猕猴的纹状皮层，以AAV1作为病毒载体，用基因编码的荧光钙传感器memTNXL转染皮质神经元。通过构建一个非常坚固和稳定的装置，将双光子扫描显微镜和猴子的头部放在一起，单个神经元在高倍率下长时间成像，运动伪影最小，持续时间长达10个月。在高倍镜下获得单个神经元的结构图像。当猴子执行注视任务时，测量了视觉刺激期间钙的变化。总的来说，在234个标记和成像的神经元中，有18.8%的神经元获得了功能反应和定向调谐曲线。这表明双光子扫描显微镜可以成功地在行为灵长类动物中获得。
Two-photon scanning microscopy has advanced our understanding of neural signaling in non-mammalian species and mammals. Various developments are needed to perform two-photon scanning microscopy over prolonged periods in non-human primates performing a behavioral task. In striate cortex in two macaque monkeys, cortical neurons were transfected with a genetically encoded fluorescent calcium sensor, memTNXL, using AAV1 as a viral vector. By constructing an extremely rigid and stable apparatus holding both the two-photon scanning microscope and the monkey's head, single neurons were imaged at high magnification for prolonged periods with minimal motion artifacts for up to ten months. Structural images of single neurons were obtained at high magnification. Changes in calcium during visual stimulation were measured as the monkeys performed a fixation task. Overall, functional responses and orientation tuning curves were obtained in 18.8% of the 234 labeled and imaged neurons. This demonstrated that the two-photon scanning microscopy can be successfully obtained in behaving primates.
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影响因子：
2.5
作者：
EVARTS, EV
通讯作者：
EVARTS, EV
DOI：
10.1038/mt.2008.166
发表时间：
2008-10
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.88.24.11559
发表时间：
1991-12-01
影响因子：
11.1
作者：
GRINVALD, A;FROSTIG, RD;BARTFELD, E
通讯作者：
BARTFELD, E
影响因子：
25
作者：
Guerrero, G;Reiff, DF;Isacoff, EY
通讯作者：
Isacoff, EY
影响因子：
56.9
作者：
Buschman, Timothy J.;Miller, Earl K.
通讯作者：
Miller, Earl K.