Phosphorus Metabolism Monitored by 31P NMR in Juvenile Sea Scallop (Placopecten magellanicus) Overwintering in Pearl Nets at a Nova Scotian Aquaculture Site

Phosphorus Metabolism Monitored by 31P NMR in Juvenile Sea Scallop (Placopecten magellanicus) Overwintering in Pearl Nets at a Nova Scotian Aquaculture Site
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通过 31P NMR 监测在新斯科舍水产养殖场珍珠网中越冬的幼海扇贝 (Placopecten magellanicus) 的磷代谢

DOI:
10.1139/f94-212
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发表时间:
1994
影响因子:
2.4
通讯作者:
J. Walter
J. Walter
中科院分区:
农林科学2区
文献类型:
--
作者:
A. E. Jackson;A. Defreitas;L. Hooper;A. Mallet;J. Walter

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利用~(31)P核磁共振(NMR)技术研究了新斯科舍省养殖场的麦哲伦扇贝(Placopecten magellanicus)幼贝体内磷代谢。NMR可见精氨酸磷酸(Arg P)的浓度从夏季到冬季下降,而无机磷酸盐(Pi)的水平增加和腺苷三磷酸(ATP)的水平保持相对恒定。我们的结果表明,在使用磷酸原Arg P补充ATP的扇贝中,Arg P与Pi的比率比称为腺苷酸能荷的腺苷酸浓度比率更好地指示能量状态。扇贝收集在秋季和冬季,当暴露于缺氧条件下,表现出明显的下降,精氨酸P与Pi的增加和ATP水平的降低,而这些保持相对不变的扇贝收集在夏季。模拟冬季条件下保持扇贝显示较低水平的精氨酸P与营养不良,和响应。
31P Nuclear magnetic resonance (NMR) spectroscopy has been used to examine in vivo phosphorus metabolism in juvenile sea scallop (Placopecten magellanicus) from an aquaculture site in Nova Scotia. NMR-visible arginine phosphate (Arg P) concentrations declined from summer to winter whereas inorganic phosphate (Pi) levels increased and those of adenosine triphosphate (ATP) remained relatively constant. Our results indicate that, in the scallop that uses the phosphagen Arg P to replenish ATP, the ratio of Arg P to Pi is a better indicator of energetic status than is the ratio of adenylate concentrations known as adenylate energy charge. Scallops collected in fall and winter, when exposed to hypoxic conditions, showed pronounced decreases in Arg P associated with increases in Pi and decreases in ATP levels whereas these remained relatively unchanged in scallops collected in summer. Scallops maintained under simulated winter conditions showed lower levels of Arg P associated with poor nutrition, and a response...
通过 NMR 监测细胞代谢。
DOI: 10.1016/0304-4173(81)90005-7
发表时间: 1981
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影响因子: --
作者:
Roberts,JK;Jardetzky,O
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发表时间: 1991
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影响因子: --
作者:
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