Rice ASR1 and ASR5 are complementary transcription factors regulating aluminium responsive genes.
Rice ASR1 and ASR5 are complementary transcription factors regulating aluminium responsive genes.
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水稻ASR1和ASR5是调节铝反应基因的互补转录因子。
DOI：
10.1111/pce.12655
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发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Margis-Pinheiro M
中科院分区：
文献类型：
--
作者：
Arenhart RA;Schunemann M;Bucker Neto L;Margis R;Wang ZY;Margis-Pinheiro M
水稻是最耐受铝毒害的粮食作物，铝毒害是世界范围内粮食生产的极限胁迫。这种耐铝性是部分由转录因子ASR5触发的综合机制的结果。ASR是一种双靶蛋白，在细胞质中以伴侣形式参与，在细胞核中作为转录因子参与。此外，这些蛋白质对包括盐、干旱和铝在内的生物和非生物胁迫做出反应。携带沉默ASR基因的水稻植株对铝高度敏感。ASR5是一种与铝反应基因中特定的顺式元件结合并调节其表达的蛋白质。由于在沉默的水稻植株中发现的铝敏感表型可能是由于ASR1和ASR5的相互沉默所致，因此我们研究了ASR5的特异性沉默的效果。人工沉默ASR5的植物由于ASR1的诱导而呈现出非转化的表型。ASR1与ASR5具有相同的亚细胞定位，与ASR5顺式调控元件结合，以非优先的方式调控ASR5调控基因，在一定条件下可能取代ASR5。我们的结果表明，ASR1和ASR5协同互补地调节铝诱导的基因表达。
Rice is the most tolerant staple crop to aluminum (Al) toxicity, which is a limiting stress for grain production worldwide. This Al tolerance is the result of combined mechanisms that are triggered in part by the transcription factor ASR5. ASRs are dual target proteins that participate as chaperones in the cytoplasm and as transcription factors in the nucleus. Moreover, these proteins respond to biotic and abiotic stresses, including salt, drought and Al. Rice plants with silenced ASR genes are highly sensitive to Al. ASR5, a well-characterized protein, binds to specific cis elements in Al responsive genes and regulates their expression. Because the Al sensitive phenotype found in silenced rice plants could be due to the mutual silencing of ASR1 and ASR5, we investigated the effect of the specific silencing of ASR5. Plants with artificial microRNA silencing of ASR5 present a non-transformed phenotype in response to Al due to the induction of ASR1. ASR1 has the same subcellular localization as ASR5, binds to ASR5 cis-regulatory elements, regulates ASR5 regulated genes in a non-preferential manner and might replace ASR5 under certain conditions. Our results indicate that ASR1 and ASR5 act in concert and complementarily to regulate gene expression in response to Al.
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