A barrel-related interneuron in layer 4 of rat somatosensory cortex with a high intrabarrel connectivity.
A barrel-related interneuron in layer 4 of rat somatosensory cortex with a high intrabarrel connectivity.
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DOI：
10.1093/cercor/bht263
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Feldmeyer D
中科院分区：
文献类型：
--
作者：
Koelbl C;Helmstaedter M;Lübke J;Feldmeyer D
采用膜片钳记录和同步生物胞素填充研究了桶状皮质第4层（L4）中快速尖峰（FS）、小清蛋白（PV）阳性中间神经元和兴奋性棘神经元之间的突触连接。FS L4中间神经元的三个不同的集群进行了鉴定的基础上，其轴突形态相对于桶柱，这表明这些神经元不构成一个同质的中间神经元的人口。一种L4 FS中间神经元类型具有严格限制于L4桶的轴突结构域，因此被命名为“桶限制抑制性中间神经元”（BIn）。BIns与L4棘状神经元建立了可靠的抑制性突触连接，连接率高达67%，其中69%是相互的。这些连接处的单一IPSP的平均振幅为0.9 ± 0.8 mV，振幅变化很小，短时程突触抑制较弱。我们发现平均3.7 ± 1.3个假定的抑制性突触接触，不限于体周区。总之，我们的特点是一种新型的桶皮质中间神经元的主要丘脑受体层4形成致密的突触网络与L4多刺神经元。这些网络构成了一个有效的和强大的抑制反馈系统，这可能有助于快速重置桶微电路后的感觉激活。
Synaptic connections between identified fast-spiking (FS), parvalbumin (PV)-positive interneurons, and excitatory spiny neurons in layer 4 (L4) of the barrel cortex were investigated using patch-clamp recordings and simultaneous biocytin fillings. Three distinct clusters of FS L4 interneurons were identified based on their axonal morphology relative to the barrel column suggesting that these neurons do not constitute a homogeneous interneuron population. One L4 FS interneuron type had an axonal domain strictly confined to a L4 barrel and was therefore named “barrel-confined inhibitory interneuron” (BIn). BIns established reliable inhibitory synaptic connections with L4 spiny neurons at a high connectivity rate of 67%, of which 69% were reciprocal. Unitary IPSPs at these connections had a mean amplitude of 0.9 ± 0.8 mV with little amplitude variation and weak short-term synaptic depression. We found on average 3.7 ± 1.3 putative inhibitory synaptic contacts that were not restricted to perisomatic areas. In conclusion, we characterized a novel type of barrel cortex interneuron in the major thalamo-recipient layer 4 forming dense synaptic networks with L4 spiny neurons. These networks constitute an efficient and powerful inhibitory feedback system, which may serve to rapidly reset the barrel microcircuitry following sensory activation.
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通讯作者：
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