Real-time PCR assay for discrimination of Plasmodium ovale curtisi and Plasmodium ovale wallikeri in the Ivory Coast and in the Comoros Islands.
Real-time PCR assay for discrimination of Plasmodium ovale curtisi and Plasmodium ovale wallikeri in the Ivory Coast and in the Comoros Islands.
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DOI：
10.1186/1475-2875-11-307
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发表时间：
2012-09-04
期刊：
影响因子：
3
通讯作者：
Parzy D
中科院分区：
文献类型：
--
作者：
Bauffe F;Desplans J;Fraisier C;Parzy D
卵形疟原虫是感染人类的五种疟疾之一。最近的数据表明，这种被忽视的物种的名称掩盖了两种不同的基因型，也称为柯蒂斯和沃里克里。一些作者指出，这些物种可能是同域分布的。这两个亚种不能通过显微镜技术和疟疾快速诊断测试来区分。这种诊断缺陷是低寄生虫血症、抗原多态性和缺乏抗体性能的结果，需要使用测序技术。一种准确、简便的判别检测方法是必要的。一种新的分子检测方法被开发出来，可以很容易地鉴定出两种基因型的卵形疟原虫。利用这一工具，研究了90份经分子生物学技术确认含有卵形疟原虫的血液样本，这些样本是从输入性疟疾患者身上获得的。新的标记物在良好的基因分型样品上得到验证。对主要从科特迪瓦和科摩罗群岛进口的90份卵圆藻样品进行了基因型鉴定。这两个亚种的分布描述了大量的样本，并表明这两种基因型存在于所研究的国家。这项工作首次证实了这两个物种在同一个国家的存在，在象牙海岸和科摩罗群岛。更好的卵圆假单胞菌类型的基因分型可以改善每种类型的临床病理生理学的更好表征。
Plasmodium ovale is one of the five malaria species infecting humans. Recent data have shown that the name of this neglected species masks two distinct genotypes also called curtisi and wallikeri. Some authors show that these species could be sympatric. These two subspecies are not differentiated by microscopy techniques and malaria rapid diagnostic tests. This diagnostic defect is the result of low parasitaemia, antigenic polymorphism and absence of antibodies performance and requires the use of sequencing techniques. An accurate and easy discrimination detection method is necessary. A new molecular assay was developed to easily identify the two genotypes of P. ovale. This tool allowed the study of 90 blood samples containing P. ovale, confirmed by molecular biology techniques, which were obtained from patients with imported malaria. The new marker was validated on well genotyped samples. The genotype of 90 P. ovale samples mainly imported from the Ivory Coast and the Comoros Islands was easily and quickly realized. The distribution of the two subspecies was described with a significant number of samples and showed that the two genotypes were present in the studied countries. This work confirms the presence of the two species in the same country for the first time, in the Ivory Coast and the Comoros Islands. A better genotyping of P. ovale types may improve a better characterization of the clinical pathophysiology for each.
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影响因子：
2.9
作者：
Brown, WM;Yowell, CA;Jagt, DLV
通讯作者：
Jagt, DLV
DOI：
10.4269/ajtmh.2008.79.391
发表时间：
2008-09-01
影响因子：
3.3
作者：
Rojo-Marcos, Gerardo;Cuadros-Gonzalez, Juan;de Pablo-Sanchez, Raul
通讯作者：
de Pablo-Sanchez, Raul
影响因子：
3.7
作者：
Duval L;Nerrienet E;Rousset D;Sadeuh Mba SA;Houze S;Fourment M;Le Bras J;Robert V;Ariey F
通讯作者：
Ariey F
影响因子：
9.4
作者：
Kawamoto, F;Miyake, H;Wataya, W
通讯作者：
Wataya, W
影响因子：
3
作者：
Cinquetti, Gael;Banal, Frederic;Graffin, Bruno
通讯作者：
Graffin, Bruno