The Genomic Basis of Color Pattern Polymorphism in the Harlequin Ladybird.
The Genomic Basis of Color Pattern Polymorphism in the Harlequin Ladybird.
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DOI：
10.1016/j.cub.2018.08.023
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发表时间：
2018-10-22
期刊：
影响因子：
--
通讯作者：
Prud'homme B
中科院分区：
文献类型：
--
作者：
Gautier M;Yamaguchi J;Foucaud J;Loiseau A;Ausset A;Facon B;Gschloessl B;Lagnel J;Loire E;Parrinello H;Severac D;Lopez-Roques C;Donnadieu C;Manno M;Berges H;Gharbi K;Lawson-Handley L;Zang LS;Vogel H;Estoup A;Prud'homme B
许多动物物种由离散的表型组成。在自然昆虫种群中，一个常见的例子是出现不同的颜色模式，这促使了丰富的生态学和遗传学研究。在多个分类群中也发现了表现离散颜色模式的黑色形式，即黑色，但其潜在的基因组基础仍然缺乏特征。在许多瓢虫物种(瓢虫科)中，成虫鞘翅上的红色和黑色斑块的空间排列在物种之间差异很大，形成了截然不同的复杂颜色图案。在小丑瓢虫Harmonia axyridis中，已经描述了200多种不同的颜色形式，经典的遗传学研究表明，这些颜色是由单个未知基因的等位基因变异造成的。在这里，我们结合了全基因组测序、基于群体的全基因组关联研究、基因表达和功能分析，以确定转录因子Pannier控制着黑素模式的多态。我们证明，羽片是形成鞘翅目上的黑色成分所必需的。Pannier的等位基因变异导致在鞘翅目上不同区域的蛋白质表达，从而决定了不同的颜色模式。Pannier等位基因之间的重组可能被Pannier顺式调控区的高度分散的∼170kb序列减少，并在不同颜色形式之间有50kb的倒置。这很可能有助于保持在自然种群中发现的独特的等位基因。因此，我们认为，在自然种群中，通过单个基因的顺式调节等位基因变异，可以产生高度可变的离散颜色形式。小丑瓢虫在自然种群中表现出不同的颜色类型，转录因子Pannier控制这种颜色多态，Pannier是形成鞘翅上黑色元素所必需的。Pannier的顺式调控区在不同颜色类型之间存在着广泛的差异，在自然种群中已描述了200多种不同的颜色形式。戈蒂埃等人。证明这种变异受转录因子Pannier控制。Pannier是产生黑色颜料所必需的，它的表达模式预示着每种颜色形式中的着色模式。
Many animal species comprise discrete phenotypic forms. A common example in natural populations of insects is the occurrence of different color patterns, which has motivated a rich body of ecological and genetic research. The occurrence of dark, i.e., melanic, forms displaying discrete color patterns is found across multiple taxa, but the underlying genomic basis remains poorly characterized. In numerous ladybird species (Coccinellidae), the spatial arrangement of black and red patches on adult elytra varies wildly within species, forming strikingly different complex color patterns. In the harlequin ladybird, Harmonia axyridis, more than 200 distinct color forms have been described, which classic genetic studies suggest result from allelic variation at a single, unknown, locus. Here, we combined whole-genome sequencing, population-based genome-wide association studies, gene expression, and functional analyses to establish that the transcription factor Pannier controls melanic pattern polymorphism in H. axyridis. We show that pannier is necessary for the formation of melanic elements on the elytra. Allelic variation in pannier leads to protein expression in distinct domains on the elytra and thus determines the distinct color patterns in H. axyridis. Recombination between pannier alleles may be reduced by a highly divergent sequence of ∼170 kb in the cis-regulatory regions of pannier, with a 50 kb inversion between color forms. This most likely helps maintain the distinct alleles found in natural populations. Thus, we propose that highly variable discrete color forms can arise in natural populations through cis-regulatory allelic variation of a single gene. The harlequin ladybird displays various color pattern forms in natural populations The transcription factor Pannier controls this color polymorphism Pannier is essential for the formation of melanic elements on the elytra The cis-regulatory regions of pannier have diverged extensively among color forms More than 200 distinct color forms have been described in natural populations of the harlequin ladybird, Harmonia axyridis. Gautier et al. show that this variation is controlled by the transcription factor Pannier. Pannier is necessary to produce black pigment, and its expression pattern prefigures the coloration pattern in each color form.
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影响因子：
64.8
作者：
Dasmahapatra, Kanchon K.;Walters, James R.;Briscoe, Adriana D.;Davey, John W.;Whibley, Annabel;Nadeau, Nicola J.;Zimin, Aleksey V.;Hughes, Daniel S. T.;Ferguson, Laura C.;Martin, Simon H.;Salazar, Camilo;Lewis, James J.;Adler, Sebastian;Ahn, Seung-Joon;Baker, Dean A.;Baxter, Simon W.;Chamberlain, Nicola L.;Chauhan, Ritika;Counterman, Brian A.;Dalmay, Tamas;Gilbert, Lawrence E.;Gordon, Karl;Heckel, David G.;Hines, Heather M.;Hoff, Katharina J.;Holland, Peter W. H.;Jacquin-Joly, Emmanuelle;Jiggins, Francis M.;Jones, Robert T.;Kapan, Durrell D.;Kersey, Paul;Lamas, Gerardo;Lawson, Daniel;Mapleson, Daniel;Maroja, Luana S.;Martin, Arnaud;Moxon, Simon;Palmer, William J.;Papa, Riccardo;Papanicolaou, Alexie;Pauchet, Yannick;Ray, David A.;Rosser, Neil;Salzberg, Steven L.;Supple, Megan A.;Surridge, Alison;Tenger-Trolander, Ayse;Vogel, Heiko;Wilkinson, Paul A.;Wilson, Derek;Yorke, James A.;Yuan, Furong;Balmuth, Alexi L.;Eland, Cathlene;Gharbi, Karim;Thomson, Marian;Gibbs, Richard A.;Han, Yi;Jayaseelan, Joy C.;Kovar, Christie;Mathew, Tittu;Muzny, Donna M.;Ongeri, Fiona;Pu, Ling-Ling;Qu, Jiaxin;Thornton, Rebecca L.;Worley, Kim C.;Wu, Yuan-Qing;Linares, Mauricio;Blaxter, Mark L.;Ffrench-Constant, Richard H.;Joron, Mathieu;Kronforst, Marcus R.;Mullen, Sean P.;Reed, Robert D.;Scherer, Steven E.;Richards, Stephen;Mallet, James;McMillan, W. Owen;Jiggins, Chris D.
通讯作者：
Jiggins, Chris D.
影响因子：
64.8
作者：
Kunte, K.;Zhang, W.;Kronforst, M. R.
通讯作者：
Kronforst, M. R.
影响因子：
2.1
作者：
Michie, L. J.;Mallard, F.;Jiggins, F. M.
通讯作者：
Jiggins, F. M.
影响因子：
3.3
作者：
Hivert, Valentin;Leblois, Raphael;Vitalis, Renaud
通讯作者：
Vitalis, Renaud
影响因子：
3.3
作者：
Bidon T;Schreck N;Hailer F;Nilsson MA;Janke A
通讯作者：
Janke A