Individual stripe regulatory elements in the Drosophila hairy promoter respond to maternal, gap, and pair-rule genes.
Individual stripe regulatory elements in the Drosophila hairy promoter respond to maternal, gap, and pair-rule genes.
复制标题
果蝇毛状启动子中的单个条纹调控元件对母体基因、间隙基因和配对规则基因做出反应。
作者：
G. Riddihough;D. Ish
对规则基因hairy (h)的条纹表达在果蝇的分节调控中起核心作用。我们使用h- lacz报告基因融合来描绘驱动单个条纹表达的h序列。我们发现14kb的5'侧DNA在胚皮胚胎中指导7条lacZ条纹的表达。在这个区域内，我们发现了表达单个条纹1、5、6和7所需的离散序列，以及在条纹2域中活跃的分散元素。只有stripe1元素在精确的h条带中指导lacZ表达式；条纹5、6和7相对于它们的h对应体移位了一到两个细胞。这些结果表明调控序列分散在h启动子内。我们已经确定了lacZ条纹对母系、间隙和成对规则基因突变的敏感性。我们的研究结果表明，不同但重叠的间隙基因亚群调节每个条纹，激活和抑制在条纹模式的产生中都很重要。
Striped expression of the pair-rule gene hairy (h) plays a central role in regulating segmentation in Drosophila. We have used h-lacZ reporter gene fusions to delineate h sequences that drive individual stripe expression. We show that 14 kb of 5'-flanking DNA directs expression of seven lacZ stripes in the blastoderm embryo. Within this region, we identify discrete sequences required for expression of individual stripes 1, 5, 6, and 7, and dispersed elements active in the stripe 2 domain. Only the stripe 1 element directs lacZ expression in an accurate h stripe; stripes 5, 6, and 7 are displaced by one to two cells relative to their h counterparts. These results indicate that regulatory sequences are dispersed within the h promoter. We have determined the sensitivity of the lacZ stripes to maternal, gap, and pair-rule gene mutations. Our results suggest that different but overlapping subsets of gap genes regulate each stripe and that activation and repression are both important in generating the stripe pattern.
DOI：
--
发表时间：
1990-02
期刊：
The New biologist
影响因子：
--
作者：
M. Karin
通讯作者：
M. Karin
影响因子：
10.5
作者：
KANIA, MA;BONNER, AS;GERGEN, JP
通讯作者：
GERGEN, JP