Mass spectrometry based proteomic studies on viruses and hosts--a review.
Mass spectrometry based proteomic studies on viruses and hosts--a review.
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基于质谱的病毒和宿主研究 - 综述。
DOI：
10.1016/j.aca.2011.06.045
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发表时间：
2011-09-30
影响因子：
6.2
通讯作者：
Tang K
中科院分区：
文献类型：
--
作者：
Zheng J;Sugrue RJ;Tang K
。a)背景，RSV感染细胞的扫描电镜照片显示，病毒感染细胞表面有病毒丝出芽。b)插入顶部的MS谱表示消化的RSV病毒颗粒的特征。c)在底部插入RSV感染的细胞，用免疫荧光显微镜成像，红色代表病毒丝；绿色为HSP90；黄色染色表示两种抗原在病毒丝内共定位。►介绍了病毒和宿主蛋白质组学研究的最新进展。TAP， IP， SILAC， ICAT和iTRAQ便于样品富集和定量。►讨论临床重要病毒与宿主的相互作用。►功能验证对于确认鉴定的蛋白质的作用至关重要。在21世纪的蛋白质组学研究中，病毒学领域仍然被认为是一个巨大的挑战，主要来自三个方面，即研究具有意外变异的病毒的复杂蛋白质组，开发更精确的分析技术，以及了解病毒的发病机制和病毒与宿主的相互作用动力学。这些领域的进展将有助于疫苗设计和抗病毒药物的发现。基于质谱的蛋白质组学已经显示出非凡的能力，不仅可以精确地识别病毒和细胞蛋白在病毒感染时的功能、结构和动态变化，而且可以使我们检测重要的途径蛋白。此外，许多与质谱相结合的分离纯化技术和定量策略可以显著提高质谱检测低丰度蛋白质的灵敏度，补充病毒蛋白质组的库存，扩大蛋白质-蛋白质相互作用图谱。然而，只有一小部分动物和植物的传染性病毒使用这种方法进行了研究。随着越来越多的病毒和宿主基因组被测序，基于质谱的蛋白质组学正在成为病毒学不可或缺的工具。在本文中，我们简要回顾了目前的技术及其在研究选定的病毒和宿主方面的应用。
. a) In the background, scanning electron micrograph of RSV infected cells reveals viral filaments budding from the surface of virus infected cells. b) Inserted at the top, MS spectrum represents the characterization of the digested RSV virus particles. c) Inserted at the bottom, RSV infected cells were imaged using immunofluorescence microscopy: red represents virus filaments; green is HSP90; yellow staining represents co-localization of both antigens within the virus filaments. ► The current proteomic researches on viruses and hosts are described. ► TAP, IP, SILAC, ICAT, and iTRAQ facilitate sample enrichment and quantification. ► Clinically important viruses are discussed on their interactions with hosts. ► Functional validation is essential to confirm the roles of the identified proteins. In terms of proteomic research in the 21st century, the realm of virology is still regarded as an enormous challenge mainly brought by three aspects, namely, studying on the complex proteome of the virus with unexpected variations, developing more accurate analytical techniques as well as understanding viral pathogenesis and virus–host interaction dynamics. Progresses in these areas will be helpful to vaccine design and antiviral drugs discovery. Mass spectrometry based proteomics have shown exceptional display of capabilities, not only precisely identifying viral and cellular proteins that are functionally, structurally, and dynamically changed upon virus infection, but also enabling us to detect important pathway proteins. In addition, many isolation and purification techniques and quantitative strategies in conjunction with MS can significantly improve the sensitivity of mass spectrometry for detecting low-abundant proteins, replenishing the stock of virus proteome and enlarging the protein–protein interaction maps. Nevertheless, only a small proportion of the infectious viruses in both of animal and plant have been studied using this approach. As more virus and host genomes are being sequenced, MS-based proteomics is becoming an indispensable tool for virology. In this paper, we provide a brief review of the current technologies and their applications in studying selected viruses and hosts.
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影响因子：
3.4
作者：
Deutsch, Eric W.;Mendoza, Luis;Shteynberg, David;Farrah, Terry;Lam, Henry;Tasman, Natalie;Sun, Zhi;Nilsson, Erik;Pratt, Brian;Prazen, Bryan;Eng, Jimmy K.;Martin, Daniel B.;Nesvizhskii, Alexey I.;Aebersold, Ruedi
通讯作者：
Aebersold, Ruedi
影响因子：
3.8
作者：
Chen, Li Ming;Tran, Bich Ngoc;Hew, Choy-Leong
通讯作者：
Hew, Choy-Leong
影响因子：
3.7
作者：
Deshmane, Satish L.;Mukerjee, Ruma;Sawaya, Bassel E.
通讯作者：
Sawaya, Bassel E.
影响因子：
64.5
作者：
AIKEN, C;KONNER, J;TRONO, D
通讯作者：
TRONO, D
影响因子：
5.4
作者：
ANDERSON, SJ;LENBURG, M;GARCIA, JV
通讯作者：
GARCIA, JV