Thermal stability of DNA functionalized gold nanoparticles.
Thermal stability of DNA functionalized gold nanoparticles.
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DOI：
10.1021/bc300687z
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发表时间：
2013-11-20
影响因子：
4.7
通讯作者：
Le XC
中科院分区：
文献类型：
--
作者：
Li F;Zhang H;Dever B;Li XF;Le XC
DNA 功能化金纳米粒子 (DNA-AuNP) 的治疗用途在疾病诊断和治疗方面显示出巨大的潜力和令人兴奋的机会。在热应激条件下保持 DNA 寡核苷酸和金纳米粒子之间的稳定结合是任何实际应用的关键方面之一。我们系统地研究了有机硫锚定基团和堆积密度对 DNA-AuNPs 热稳定性的影响。使用荧光测定法来确定从 DNA-AuNPs 中释放 DNA 分子的动力学，当比较用不同锚定基团构建的 DNA-AuNPs 时，我们观察到温度诱导和化学诱导的 DNA 从 DNA-AuNPs 释放之间存在相反的趋势。具体来说，与环状二硫化物形成的双齿Au-S键的热稳定性低于与硫醇或无环二硫化物形成的双齿Au-S键。然而，在竞争性硫醇（巯基己醇或二硫苏糖醇）的处理下，相同的双齿 Au-S 键在化学上更加稳定。 AuNP 上的 DNA 堆积密度影响 DNA-AuNP 在 37 °C 下的热稳定性，但当温度升高到 85 °C 时，这种影响最小。随着对这些结果的进一步了解，我们能够设计一种策略，通过多个硫醇锚将双链 DNA 与 AuNP 缀合来增强 DNA-AuNP 的稳定性。
Therapeutic uses of DNA functionalized gold nanoparticles (DNA-AuNPs) have shown great potential and exciting opportunities for disease diagnostics and treatment. Maintaining stable conjugation between DNA oligonucleotides and gold nanoparticles under thermally stressed conditions is one of the critical aspects for any of the practical applications. We systematically studied the thermal stability of DNA-AuNPs as affected by organosulfur anchor groups and packing densities. Using a fluorescence assay to determine the kinetics of releasing DNA molecules from DNA-AuNPs, we observed an opposite trend between the temperature-induced and chemical-induced release of DNA from DNA-AuNPs when comparing the DNA-AuNPs that were constructed with different anchor groups. Specifically, the bidentate Au–S bond formed with cyclic disulfide was thermally less stable than those formed with thiol or acyclic disulfide. However, the same bidentate Au–S bond was chemically more stable under the treatment of competing thiols (mercaptohexanol or dithiothreitol). DNA packing density on AuNPs influenced the thermal stability of DNA-AuNPs at 37 °C, but this effect was minimum as temperature increased to 85 °C. With the improved understanding from these results, we were able to design a strategy to enhance the stability of DNA-AuNPs by conjugating double-stranded DNA to AuNPs through multiple thiol anchors.
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