Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes.
Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes.
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乳头状瘤病毒相关细胞系对载姜黄素脂质体光动力疗法的敏感性。
DOI：
10.3390/cancers12113278
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发表时间：
2020-11-05
期刊：
影响因子：
5.2
通讯作者：
Mandic R
中科院分区：
文献类型：
--
作者：
Ambreen G;Duse L;Tariq I;Ali U;Ali S;Pinnapireddy SR;Bette M;Bakowsky U;Mandic R
在全球范围内，乳头状瘤病毒相关癌症的负担很高。全世界约5%的癌症是由人乳头瘤病毒（HPV）引起的。光动力疗法（PDT）被认为是治疗癌症的一种有用的治疗选择，特别是那些靠近组织表面的癌症，因为与传统治疗策略相比，它通常耐受性好，侵入性小，严重并发症的风险较低。PDT需要光敏剂、特定波长的光和组织氧的组合。在本研究中，我们检测了PDT与姜黄素（脂质体）为基础的光敏剂在三种乳头瘤病毒相关细胞系中的有效性。姜黄素脂质体PDT可抑制肿瘤细胞的增殖、细胞迁移和集落形成。结果表明，姜黄素包封脂质体联合PDT可能是治疗乳头状瘤病毒相关肿瘤的有用工具。光动力疗法（PDT）是一种微创治疗方法，用于治疗各种医疗条件和癌症疾病，涉及光、光敏物质和氧气。姜黄素是一种天然化合物，具有抗肿瘤活性，可作为PDT的光敏剂。对于脂质体封装的姜黄素是否有助于提高这种化合物的疗效、稳定性和生物利用度，目前所知甚少。本研究探讨了载姜黄素脂质体与PDT联合使用的体外效果。用姜黄素浓度为0-100µmol/L的载姜黄素脂质体处理3株乳头瘤病毒相关细胞系4小时，然后在457 nm（蓝色）照射45、136和227 s，照射强度为220.2 W/m2 (100 mA)，对应于1、3和5 J·cm−2。24小时后，采用MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑）、SYTO9/PI（碘化丙啶）、Annexin V-FITC（异硫氰酸荧光素）/PI、克隆生存和划伤（伤口愈合）试验评估治疗的生物学结果。载姜黄素脂质体的光激活导致菌落形成和迁移能力的显著减少，以及肿瘤细胞死亡的增加。结果表明，姜黄素脂质体与PDT的结合可能是治疗乳头瘤病毒相关恶性肿瘤的有用工具。
Globally, the burden of papilloma virus-associated cancers is high. About 5% of all cancers worldwide are caused by the human papillomavirus (HPV). Photodynamic therapy (PDT) is considered as a useful therapeutic option to treat cancers, particularly those near the tissue surface, since it is typically well tolerated and less invasive with a lower risk of severe complications as compared to conventional treatment strategies. PDT requires the combination of a photosensitizer, light of a specific wavelength, and tissue oxygen. In the present study, we examined the effectiveness of PDT together with a curcumin (liposome)-based photosensitizer in three papilloma virus-associated cell lines. PDT with curcumin liposomes could inhibit proliferation, cell migration, and colony formation of the tested tumor cells. The results suggest that curcumin-encapsulated liposomes in conjunction with PDT could be a useful tool for the treatment of papilloma virus-associated tumors. Photodynamic therapy (PDT) is a minimally invasive therapeutic approach used in the treatment of various medical conditions and cancerous diseases, involving light, a photosensitizing substance, and oxygen. Curcumin, a naturally occurring compound, carries antitumor activities and potentially could be exploited as a photosensitizer in PDT. Only little is known about liposomal-encapsulated curcumin that could help in increasing the efficacy, stability, and bioavailability of this compound. This study investigates the in vitro effects of curcumin-loaded liposomes in combination with PDT. Three papilloma virus-associated cell lines were treated with curcumin-loaded liposomes corresponding to a curcumin concentration of 0–100 µmol/L for 4 h followed by illumination at 457 nm (blue) for 45, 136, and 227 s at a fluence of 220.2 W/m2 (100 mA) corresponding to 1, 3 and 5 J·cm−2. After 24 h, the biological outcome of the treatment was assessed with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), SYTO9/PI (propidium iodide), Annexin V-FITC (fluorescein isothiocyanate)/PI, clonogenic survival, and scratch (wound closure) assays. Photoactivation of curcumin-loaded liposomes led to a significant reduction in colony formation and migratory abilities, as well as to an increase in tumor cell death. The results point to the combination of curcumin-loaded liposomes with PDT as a potentially useful tool for the treatment of papillomavirus-associated malignancies.
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DOI：
10.3322/caac.20114
发表时间：
2011-07
期刊：
CA: a cancer journal for clinicians
影响因子：
--
作者：
Agostinis P;Berg K;Cengel KA;Foster TH;Girotti AW;Gollnick SO;Hahn SM;Hamblin MR;Juzeniene A;Kessel D;Korbelik M;Moan J;Mroz P;Nowis D;Piette J;Wilson BC;Golab J
通讯作者：
Golab J
DOI：
10.1080/21691401.2020.1725023
发表时间：
2020-01-01
影响因子：
5.8
作者：
do Bonfim, Caroline Measso;Monteleoni, Leticia Figueiredo;Rahal, Paula
通讯作者：
Rahal, Paula
影响因子：
34.3
作者：
Arbyn, Marc;Weiderpass, Elisabete;Bray, Freddie
通讯作者：
Bray, Freddie
影响因子：
--
作者：
Akbarzadeh A;Rezaei-Sadabady R;Davaran S;Joo SW;Zarghami N;Hanifehpour Y;Samiei M;Kouhi M;Nejati-Koshki K
通讯作者：
Nejati-Koshki K
影响因子：
2.7
作者：
Kreider, J W;Bartlett, G L
通讯作者：
Bartlett, G L