Erythrocytes inhibit ligament fibroblast proliferation in a collagen scaffold.
Erythrocytes inhibit ligament fibroblast proliferation in a collagen scaffold.
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红细胞抑制胶原支架中的韧带成纤维细胞增殖。
DOI：
10.1002/jor.21321
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发表时间：
2011-09
影响因子：
2.8
通讯作者：
Murray, Martha M.
中科院分区：
文献类型：
--
作者：
Harrison, Sophia L.;Vavken, Patrick;Murray, Martha M.
在这项工作中，我们假设临时支架中的红细胞浓度会对早期伤口愈合中发生的三个主要生物学过程产生重大影响。在含有成纤维细胞和亚生理（1 × 108红细胞/ml）、生理（1 × 109红细胞/ml）和生理上（1 × 1010红细胞/ml）浓度的红细胞的胶原凝胶中，测量ACL成纤维细胞增殖、胶原生成和支架收缩。只含血小板贫乏血浆的成纤维细胞种子凝胶作为对照组。所有凝胶分别培养1、14和21天。采用DNA、酶联免疫吸附法测定前胶原蛋白和支架尺寸来量化上述三个伤口愈合参数。红细胞浓度低于全血时，对成纤维细胞增殖和支架收缩的刺激大于红细胞浓度与全血相似时（p <0.027; p <0.03）。红细胞浓度高于全血时，可刺激成纤维细胞胶原生成（p <0.009），限制支架收缩（p <0.031）。进一步研究红细胞在早期临时支架中的作用是必要的。
In this work, we hypothesized that the concentration of erythrocytes in a provisional scaffold would have a significant effect on three of the major biological processes occurring in early wound healing. ACL fibroblast proliferation, collagen production, and scaffold contraction were measured in collagen gels containing fibroblasts and erythrocytes in subphysiologic (1 × 108 erythrocytes/ml), physiologic (1 × 109 erythrocytes/ml), and supraphysiologic (1 × 1010 erythrocytes/ml) concentrations. Fibroblast-seeded gels containing only platelet-poor plasma were used as a control group. All gels were cultured for 1, 14, and 21 days. DNA, ELISA for procollagen and scaffold size measurements were used to quantify the three above parameters of wound healing. Samples with concentrations of erythryocytes lower than that in whole blood stimulated greater fibroblast proliferation and scaffold contraction than those with erythrocyte concentrations similar to that in whole blood (p <0.027; p <0.03). Increasing the erythrocyte concentration over that in the whole blood stimulated fibroblast collagen production (p <0.009) and limited scaffold contraction (p <0.031). Further work examining the role of the erythrocyte in the early provisional scaffold is warranted.
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影响因子：
3.8
作者：
Xerogeanes, JW;Fox, RJ;Woo, SLY
通讯作者：
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作者：
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作者：
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