Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease.
Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease.
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DOI：
10.1016/j.vaccine.2016.04.020
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发表时间：
2016-06-24
期刊：
影响因子：
5.5
通讯作者：
Payton ME
中科院分区：
文献类型：
--
作者：
Fulton RW;d'Offay JM;Landis C;Miles DG;Smith RA;Saliki JT;Ridpath JF;Confer AW;Neill JD;Eberle R;Clement TJ;Chase CC;Burge LJ;Payton ME
本研究调查了饲养场中牛呼吸道疾病（BRD）病例中的病毒，包括牛疱疹病毒-1（BoHV-1）、牛病毒性腹泻病毒（BVDV）、牛呼吸道合胞病毒（BRSV）、牛冠状病毒（BoCV）和副流感病毒-3（PI 3V）。从接受初始BRD治疗的114头牛中采集鼻拭子。处理包括改良活病毒（MLV）接种。共121例病例中包括7例BRD尸检病例。首次采样前的平均进食天数为14.9天。通过凝胶PCR（G-PCR）、定量PCR（qPCR）和定量真实的时间逆转录酶PCR（qRT-PCR）和病毒培养检测拭子和组织匀浆。87/114（76.3%）拭子至少一次检测至少一种病毒呈阳性。所有尸检病例均为至少一种病毒阳性。在121例病例中，阳性包括18/121（14.9%）BoHV-1; 19/121（15.7%）BVDV; 76/121（62.8%）BoCV; 11/121（9.1%）BRSV;和10/121（8.3%）PI 3V。对于鼻拭子，G-PCR（5种病毒）检出44/114（38.6%）; q-PCR和qRT-PCR（4种病毒）检出81/114（71.6%）;病毒分离检出40/114（35.1%）。大多数人只对一个或两个测试呈阳性，但不是所有三个测试。尸检病例阳性：5/7例G-PCR、5/7例q-PCR和qRT-PCR，细胞培养均为阳性。在某些情况下，G-PCR和两种真实的时间PCR在培养阳性样品中均为BoHV-1、BVDV和PI 3 V阴性。PCR未区分BoHV-1、BVDV和PI 3V的田间和疫苗株。然而，根据测序和分析，BoHV-1、BoCV、BVDV和PI 3V培养物阳性的田间和疫苗株中，11/18（61.1%）BoHV-1分离株、6/17（35.3%）BVDV分离株和1/10（10.0%）PI 3V被鉴定为疫苗。BRSV仅通过PCR检测鉴定。由于基于分子的检测和病毒分离不能将田间与疫苗株分开，因此实验室检测的解释是适当的。使用测序进行的额外检测似乎适用于鉴定疫苗株。
This study investigated viruses in bovine respiratory disease (BRD) cases in feedlots, including bovine herpesvirus-1 (BoHV-1), bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), bovine coronaviruses (BoCV) and parainfluenza-3 virus (PI3V). Nasal swabs were collected from 114 cattle on initial BRD treatment. Processing included modified live virus (MLV) vaccination. Seven BRD necropsy cases were included for 121 total cases. Mean number of days on feed before first sample was 14.9 days. Swabs and tissue homogenates were tested by gel based PCR (G-PCR), quantitative-PCR (qPCR) and quantitative real time reverse transcriptase PCR (qRT-PCR) and viral culture. There were 87/114 (76.3%) swabs positive for at least one virus by at least one test. All necropsy cases were positive for at least one virus. Of 121 cases, positives included 18/121 (14.9%) BoHV-1; 19/121 (15.7%) BVDV; 76/121 (62.8%) BoCV; 11/121 (9.1%) BRSV; and 10/121 (8.3%) PI3V. For nasal swabs, G-PCR (5 viruses) detected 44/114 (38.6%); q-PCR and qRT-PCR (4 viruses) detected 81/114 (71.6%); and virus isolation detected 40/114 (35.1%). Most were positive for only one or two tests, but not all three tests. Necropsy cases had positives: 5/7 G-PCR, 5/7 q-PCR and qRT-PCR, and all were positive by cell culture. In some cases, G-PCR and both real time PCR were negative for BoHV-1, BVDV, and PI3V in samples positive by culture. PCR did not differentiate field from vaccines strains of BoHV-1, BVDV, and PI3V. However based on sequencing and analysis, field and vaccine strains of culture positive BoHV-1, BoCV, BVDV, and PI3V, 11/18 (61.1%) of BoHV-1 isolates, 6/17 (35.3%) BVDV isolates, and 1/10 (10.0%) PI3V identified as vaccine. BRSV was only identified by PCR testing. Interpretation of laboratory tests is appropriate as molecular based tests and virus isolation cannot separate field from vaccine strains. Additional testing using sequencing appears appropriate for identifying vaccine strains.
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影响因子：
1.5
作者：
Mahlum, CE;Haugerud, S;Faaberg, KS
通讯作者：
Faaberg, KS
影响因子：
1.5
作者：
Fulton, Robert W.;Blood, K. Shawn;Reek, Amy
通讯作者：
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影响因子：
3.1
作者：
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通讯作者：
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影响因子：
9.4
作者：
Klima, Cassidy L.;Zaheer, Rahat;McAllister, Tim A.
通讯作者：
McAllister, Tim A.
DOI：
10.2460/javma.228.4.578
发表时间：
2006-02-15
影响因子：
1.9
作者：
Fulton, RW;Hessman, B;Funk, RA
通讯作者：
Funk, RA