BAC Recombineering of the Agouti Loci from Spotted Gar and Zebrafish Reveals the Evolutionary Ancestry of Dorsal-Ventral Pigment Asymmetry in Fish.
BAC Recombineering of the Agouti Loci from Spotted Gar and Zebrafish Reveals the Evolutionary Ancestry of Dorsal-Ventral Pigment Asymmetry in Fish.
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DOI：
10.1002/jez.b.22748
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Rotllant J
中科院分区：
文献类型：
--
作者：
Cal L;MegÍas M;Cerdá-Reverter JM;Postlethwait JH;Braasch I;Rotllant J
背腹色素图案的特征是腹腔浅色和背部深色，是脊椎动物身体着色中最广泛的色彩适应之一。在哺乳动物中，这种反遮蔽取决于刺豚鼠信号蛋白（ASIP）的差异表达，该蛋白驱动黑素细胞内一种黑色素的合成转换为另一种黑色素的合成。硬骨鱼共享反阴影，但这种模式是由多种类型的色素细胞的差异分布造成的，其中黑棕色黑色素细胞在背体中最丰富，反射性虹膜细胞在腹侧身体中最丰富。我们之前表明 Asip1（哺乳动物 ASIP 的鱼类直系同源物）在黑色素细胞的模式化中发挥作用。这一观察结果得出了一个令人惊讶的假设，即刺鼠可能通过调节哺乳动物和鱼类的不同机制来控制进化上保守的色素模式。为了验证这一假设，我们比较了两种辐鳍鱼：硬骨鱼斑马鱼和非硬骨鱼斑雀鱼（Lepisosteus oculatus）。通过检查 gar 中 asip1 表达的内源模式，我们证明了 asip1 表达的背腹分级分布，腹侧最高，类似于硬骨鱼。此外，在第一个报道的实验中，从斑点雀鳝中产生携带细菌人工染色体（BAC）的斑马鱼转基因品系，我们发现，两种转基因斑马鱼品系的胚胎都复制了成年斑马鱼的内源性 asip1 表达模式，表明来自这两个物种的 BAC 转基因包含成年射线鳍鱼中正常 asip1 表达所需的所有调控元件。这些实验提供的证据表明，导致对环境重要的色素模式的机制可能在硬骨鱼起源之前就已经存在。
Dorso-ventral pigment patterning, characterized by a light ventrum and a dark dorsum, is one of the most widespread chromatic adaptations in vertebrate body coloration. In mammals, this countershading depends on differential expression of agouti-signaling protein (ASIP), which drives a switch of synthesis of one type of melanin to another within melanocytes. Teleost fish share countershading, but the pattern results from a differential distribution of multiple types of chromatophores, with black-brown melanophores most abundant in the dorsal body and reflective iridophores most abundant in the ventral body. We previously showed that Asip1 (a fish ortholog of mammalian ASIP) plays a role in patterning melanophores. This observation leads to the surprising hypothesis that agouti may control an evolutionarily conserved pigment pattern by regulating different mechanisms in mammals and fish. To test this hypothesis, we compared two ray-finned fishes: the teleost zebrafish and the non-teleost spotted gar (Lepisosteus oculatus). By examining the endogenous pattern of asip1 expression in gar, we demonstrate a dorso-ventral graded distribution of asip1 expression that is highest ventrally, similar to teleosts. Additionally, in the first reported experiments to generate zebrafish transgenic lines carrying a bacterial artificial chromosome (BAC) from spotted gar, we show that both transgenic zebrafish lines embryos replicate the endogenous asip1 expression pattern in adult zebrafish, showing that BAC transgenes from both species contain all of the regulatory elements required for regular asip1 expression within adult ray-finned fishes. These experiments provide evidence that the mechanism leading to an environmentally important pigment pattern was likely in place before the origin of teleosts.
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影响因子：
4.3
作者：
Ceinos, Rosa M.;Guillot, Raul;Rotllant, Josep
通讯作者：
Rotllant, Josep
DOI：
10.1111/j.1600-0749.2004.00195.x
发表时间：
2005-02-01
期刊：
PIGMENT CELL RESEARCH
影响因子：
--
作者：
Girardot, M;Martin, J;Oulmouden, A
通讯作者：
Oulmouden, A
影响因子：
7.7
作者：
Fadeev A;Krauss J;Frohnhöfer HG;Irion U;Nüsslein-Volhard C
通讯作者：
Nüsslein-Volhard C
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
DOI：
10.1073/pnas.91.21.9760
发表时间：
1994-10-11
影响因子：
11.1
作者：
KWON, HY;BULTMAN, SJ;WOYCHIK, RP
通讯作者：
WOYCHIK, RP