Genome-wide identification, expression analysis, and functional study of the GRAS transcription factor family and its response to abiotic stress in sorghum [Sorghum bicolor (L.) Moench].
Genome-wide identification, expression analysis, and functional study of the GRAS transcription factor family and its response to abiotic stress in sorghum [Sorghum bicolor (L.) Moench].
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GRAS转录因子家族的全基因组鉴定，表达分析和功能研究及其对高粱非生物应激的反应[高粱双色（L.）Moench]。
DOI：
10.1186/s12864-021-07848-z
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发表时间：
2021-07-06
期刊：
影响因子：
4.4
通讯作者：
Cheng J
中科院分区：
文献类型：
--
作者：
Fan Y;Yan J;Lai D;Yang H;Xue G;He A;Guo T;Chen L;Cheng XB;Xiang DB;Ruan J;Cheng J
GRAS是一个重要的转录因子家族，在植物发育和非生物胁迫响应中起着重要的调控作用。自高粱基因组测序以来，大量的遗传学研究主要集中在基因组信息上。目前，对GRAS家族基因的深入鉴定和全基因组分析，特别是对两色高粱的研究还很少。根据S.双色基因组它们被命名为SbGRAS 01至SbGRAS 81，并分为13个亚家族（LISCL、DLT、OS 19、SCL 4/7、PAT 1、SHR、SCL 3、HAM-1、SCR、DELLA、HAM-2、LAS和OS 4）。SbGRAS基因在染色体上分布不均匀。根据基因和模体组成的结果，位于同一组的SbGRAS成员包含类似的内含子/外显子和模体组织。我们发现串联重复序列对高粱GRAS成员增加的贡献略大于片段重复序列。通过定量（q）RT-PCR，定量了13个SbGRAS成员在不同植物组织和在幼苗期暴露于6种非生物胁迫的植物中的表达。进一步研究了DELLA基因、GAs与小麦籽粒发育的关系。双色的多效唑处理显著增加了籽粒重，并影响了DELLA亚家族基因的表达水平。SbGRAS 03对多效唑处理最敏感，但对非生物胁迫也有很高的响应。总的来说，SbGRAs在植物发育和对非生物胁迫的响应中起着重要作用。该系统分析为进一步研究S.双色的在线版本包含补充材料，可通过10.1186/s12864-021-07848-z获得。
GRAS, an important family of transcription factors, have played pivotal roles in regulating numerous intriguing biological processes in plant development and abiotic stress responses. Since the sequencing of the sorghum genome, a plethora of genetic studies were mainly focused on the genomic information. The indepth identification or genome-wide analysis of GRAS family genes, especially in Sorghum bicolor, have rarely been studied. A total of 81 SbGRAS genes were identified based on the S. bicolor genome. They were named SbGRAS01 to SbGRAS81 and grouped into 13 subfamilies (LISCL, DLT, OS19, SCL4/7, PAT1, SHR, SCL3, HAM-1, SCR, DELLA, HAM-2, LAS and OS4). SbGRAS genes are not evenly distributed on the chromosomes. According to the results of the gene and motif composition, SbGRAS members located in the same group contained analogous intron/exon and motif organizations. We found that the contribution of tandem repeats to the increase in sorghum GRAS members was slightly greater than that of fragment repeats. By quantitative (q) RT-PCR, the expression of 13 SbGRAS members in different plant tissues and in plants exposed to six abiotic stresses at the seedling stage were quantified. We further investigated the relationship between DELLA genes, GAs and grain development in S. bicolor. The paclobutrazol treatment significantly increased grain weight, and affected the expression levels of all DELLA subfamily genes. SbGRAS03 is the most sensitive to paclobutrazol treatment, but also has a high response to abiotic stresses. Collectively, SbGRAs play an important role in plant development and response to abiotic stress. This systematic analysis lays the foundation for further study of the functional characteristics of GRAS genes of S. bicolor. The online version contains supplementary material available at 10.1186/s12864-021-07848-z.
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影响因子：
7.4
作者：
Engstrom, Eric M.;Andersen, Carl M.;Bowman, John L.
通讯作者：
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影响因子：
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影响因子：
14.9
作者：
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影响因子：
64.5
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