The Role of Intestinal Microbiota in Regulating the Metabolism of Bile Acids Is Conserved Across Vertebrates.
The Role of Intestinal Microbiota in Regulating the Metabolism of Bile Acids Is Conserved Across Vertebrates.
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肠道微生物群在调节胆汁酸代谢中的作用在脊椎动物中是保守的
DOI：
10.3389/fmicb.2022.824611
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发表时间：
2022
影响因子：
5.2
通讯作者：
Wu S
中科院分区：
文献类型：
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作者：
Xiong F;Chen S;Jakovlić I;Li W;Li M;Zou H;Wang G;Wu S
在哺乳动物中，胆汁酸(BA)浓度在很大程度上受肠道微生物区系的调节，一项研究表明，斑马鱼和小鼠对肠道微生物区系的某些代谢反应是保守的。然而，目前尚不清楚特定肠道微生物对BA代谢的影响是否在高等和低级脊椎动物(即哺乳动物和鱼类)之间保守。本研究将从草鱼肠道中分离到的弗氏柠檬酸杆菌GC01菌株添加到鱼和小鼠饲料中。我们发现，在草鱼和喂食弗劳迪线虫的小鼠中，胆汁酸谱发生了变化，特别是次级BAs的显著变化。此外，弗劳迪弧菌对脂肪代谢也有显著影响。对肝脏转录组测序数据的分析和RT-qPCR验证表明，CYP7A1基因在草鱼和小鼠中都显著上调。此外，HNF4B在草鱼中的过表达导致了CYP7A1的表达水平显著提高。总体而言，我们的结果表明，肠道微生物群对BAS的代谢在脊椎动物中是保守的。此外，特异性肠道细菌可能通过细胞色素P7A1调节胆盐的合成，HNF4B可能是鱼类BA代谢的重要调节因子。
In mammals, bile acid (BA) concentrations are regulated largely by the gut microbiota, and a study has shown that some metabolic responses to the gut microbiota are conserved between zebrafish and mice. However, it remains unknown whether the influence of specific intestinal microbes on BA metabolism is conserved between higher and lower vertebrates (i.e., mammals and fish). In the present study, Citrobacter freundii GC01 isolated from the grass carp (Ctenopharyngodon idella) intestine was supplemented to the fish and mice feed. We found the changes in the bile acid profile, especially significant changes in secondary BAs in both grass carp and mice fed on C. freundii. Also, lipid metabolism was significantly affected by C. freundii. Analysis of liver transcriptome sequencing data and validation by RT-qPCR revealed that the CYP7A1 gene was significantly up-regulated in both grass carp and mice. In addition, the overexpression of HNF4B from grass carp resulted in a significant increase in the expression level of CYP7A1. Generally, our results suggest that the metabolism of BAs by intestinal microbiota is conserved across vertebrates. Furthermore, specific intestinal bacteria may regulate the bile salt synthesis through CYP7A1 and that HNF4B might be an important regulator of BA metabolism in fish.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
14.9
作者：
Harris, MA;Clark, J;White, R
通讯作者：
White, R
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
4.2
作者：
Kong, Yunhong;Teather, Ronald;Forster, Robert
通讯作者：
Forster, Robert
影响因子：
46.9
作者：
通讯作者：
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