Relative locations of the centromere and imprinted SNRPN gene within chromosome 15 territories during the cell cycle in HL60 cells.
Relative locations of the centromere and imprinted SNRPN gene within chromosome 15 territories during the cell cycle in HL60 cells.
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HL60 细胞细胞周期期间 15 号染色体区域内着丝粒和印记 SNRPN 基因的相对位置。
DOI：
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发表时间：
2000
影响因子：
4
通讯作者：
Katsuzumi Okumura
中科院分区：
文献类型：
--
作者：
Masahiro Nogami;A. Kohda;Hiroshi Taguchi;Mitsuyoshi Nakao;Toshimichi Ikemura;Katsuzumi Okumura
印迹区域的研究提供了线索，增加了我们对基因功能调控的理解，在更高的顺序染色体域。在这里，染色体15着丝粒和印迹SNRPN基因在人髓性白血病HL 60细胞间期核的相对位置进行了比较，因为这个印迹染色体结构域的同源关联先前在淋巴细胞和淋巴母细胞中观察到。四个目标，包括15号染色体的领土，其着丝粒，SNRPN基因在这条染色体上，和细胞核，同时可视化在三维保存的细胞核中使用荧光原位杂交，这些探针的空间分布进行了分析与冷却的CCD相机解卷积系统。我们发现，优先协会SNRPN interhomologues没有发生在HL 60细胞的细胞周期，虽然这个基因表现出异步复制和单等位基因表达在这个细胞。SNRPN被发现定位在染色体区域的外围，它优先面向核膜，不像相邻的着丝粒重复。SNRPN基因和着丝粒在S期后期相互靠近，反映了随着S期的进展，这些DNA片段可能在为随后的G（2）期做准备的过程中被压缩成相同的核内亚室。我们的研究结果表明，尽管印迹基因具有与其他基因编码序列的核内定位所观察到的特征相似的特征，但印迹区域的相互识别的特征是由某些细胞调节决定的，而不是印迹基因的等位基因特异性特征所必需的。
Investigations of imprinted regions provide clues that increase our understanding of the regulation of gene functions at higher order chromosomal domains. Here, the relative positions of the chromosome 15 centromere and the imprinted SNRPN gene in interphase nuclei of human myeloid leukemia HL60 cells were compared, because the homologous association of this imprinted chromosomal domain was previously observed in lymphocytes and lymphoblasts. Four targets including the chromosome 15 territory, its centromere, the SNRPN gene on this chromosome, and the nucleus, were visualized simultaneously in three-dimensionally preserved nuclei using multicolor fluorescence in situ hybridization, and the spatial distributions of these probes were analyzed with a cooled CCD camera deconvolution system. We found that preferential association of SNRPN interhomologues did not occur during the cell cycle in HL60 cells, although this gene exhibited asynchronous replication and monoallelic expression in this cells. SNRPN was found to localize at the periphery of the chromosome territories, and it preferentially faced the nuclear membrane, unlike the adjacent centromeric repeat. The SNRPN gene and the centromere were located close to each other late in S phase, reflecting that these DNA segments may be compacted into the same intranuclear subcompartments with the progress of S phase and in course of preparation for the following G(2) phase. Our results suggest that, although an imprinted gene has features similar to those observed with intranuclear localization of other gene coding sequences, the characteristic of mutual recognition of imprinted regions is determined by certain cellular regulation, and it is not necessary for the allele-specific features of an imprinted gene.
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人类 15q 染色体着丝粒周围区域的一个大的多态性重复包含三个部分基因重复。
影响因子：
3.5
作者：
Ritchie,RJ;Mattei,MG;Lalande,M
通讯作者：
Lalande,M
影响因子：
4
作者：
J. Strouboulis;A. Wolffe
通讯作者：
J. Strouboulis;A. Wolffe
影响因子：
11.4
作者：
SELIG, S;OKUMURA, K;CEDAR, H
通讯作者：
CEDAR, H
DOI：
10.1083/jcb.108.1.1
发表时间：
1989-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nakayasu H;Berezney R
通讯作者：
Berezney R
DOI：
10.1073/pnas.85.23.9138
发表时间：
1988-12-01
影响因子：
11.1
作者：
PINKEL, D;LANDEGENT, J;GRAY, J
通讯作者：
GRAY, J