A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021.
A Severe Gastroenteritis Outbreak of Salmonella enterica Serovar Enteritidis Linked to Contaminated Egg Fried Rice, China, 2021.
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2021 年中国爆发与受污染蛋炒饭有关的肠炎沙门氏菌严重胃肠炎
DOI：
10.3389/fmicb.2021.779749
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发表时间：
2021
影响因子：
5.2
通讯作者：
Qiu S
中科院分区：
文献类型：
--
作者：
Zhang Y;Liu K;Zhang Z;Tian S;Liu M;Li X;Han Y;Zhu K;Liu H;Yang C;Liu H;Du X;Wang Q;Wang H;Yang M;Wang L;Song H;Yang H;Xiang Y;Qiu S
鸡蛋和蛋壳的沙门氏菌污染已被确定为世界范围内的公共卫生问题。在这里，我们报告了一次由肠道沙门氏菌引起的严重胃肠道症状的爆发。在中国。我们通过流行病学调查、常规实验室检测方法和全基因组测序（WGS）对疫情进行了评估。此次疫情发生在北京的一家食堂，2021年3月9日至11日期间，在该食堂用餐的324名用餐者中有225人出现胃肠道症状。本次暴发具有典型的流行病学和临床特征。在较短的潜伏期内，引起了很高的发病率（69.4%）。所有患者均出现腹泻、高热，伴有腹痛（62.3%）、恶心（50.4%）和呕吐（62.7%）。平均腹泻次数为12.4次/天，最高腹泻次数高达50次/天。平均发烧温度为39.4 ℃，最高发烧温度为42 ℃。20株S.回收了19种细菌，其中19种来自患者样品，1种来自剩余的蛋炒饭。药敏试验结果表明，20株暴发菌株的耐药模式基本一致。PFGE结果表明，所有20个菌株都具有完全相同的条带。基于WGS的系统发育分析显示，所有20个暴发菌株紧密聚在一起。因此本次食物中毒事件的致病源可能是被污染的蛋炒饭。耐药基因分析表明，本次暴发菌株均为多重耐药菌株。毒力基因分析表明，这些暴发菌株携带大量毒力基因，包括2种沙门氏菌毒力岛（SPI-1和SPI-2）。暴发菌株还携带pefABCD、rck和shdA等重要毒力基因。与暴发株处于同一进化分支的其他菌株均未检测到shdA基因。我们推测，这是这次爆发中出现严重肠胃炎症状的重要原因。本次疫情由S.卫生部建议政府应加强监测克隆菌株的流行情况，并采取措施减轻受污染鸡蛋对公共卫生构成的威胁。
Salmonella contamination of eggs and egg shells has been identified as a public health problem worldwide. Here, we reported an outbreak of severe gastrointestinal symptoms caused by Salmonella enterica serovar Enteritidis (S. enteritidis) in China. We evaluated the outbreak by using epidemiological surveys, routine laboratory testing methods, and whole genome sequencing (WGS). This outbreak occurred in a canteen in Beijing, during March 9–11, 2021, 225 of the 324 diners who have eaten at the canteen showed gastrointestinal symptoms. The outbreak had characteristical epidemiological and clinical features. It caused a very high attack rate (69.4%) in a short incubation time. All patients developed diarrhea and high fever, accompanied by abdominal pain (62.3%), nausea (50.4%), and vomiting (62.7%). The average frequency of diarrhea was 12.4 times/day, and the highest frequency of diarrhea was as high as 50 times/day. The average fever temperature was 39.4°C, and the highest fever temperature was 42°C. Twenty strains of S. enteritidis were recovered, including 19 from the patients samples, and one from remained egg fried rice. Antibiotic susceptibility test showed that the 20 outbreak strains all had the same resistance pattern. PFGE results demonstrated that all 20 strains bore completely identical bands. Phylogenetic analysis based on WGS revealed that all 20 outbreak strains were tightly clustered together. So the pathogenic source of this food poisoning incident may was contaminated egg fried rice. Resistance gene analysis showed that the outbreak strains are all multi-drug resistant strains. Virulence gene analysis indicated that these outbreak strains carried a large number of virulence genes, including 2 types of Salmonella pathogenicity islands (SPI-1 and SPI-2). Other important virulence genes were also carried by the outbreak strains, such as pefABCD, rck and shdA. And the shdA gene was not in other strains located in the same evolutionary branch as the outbreak strain. We speculated that this is a significant reason for the serious symptoms of gastroenteritis in this outbreak. This outbreak caused by S. enteritidis suggested government should strengthen monitoring of the prevalence of outbreak clone strains, and take measures to mitigate the public health threat posed by contaminated eggs.
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影响因子：
5.7
作者：
Mambu J;Virlogeux-Payant I;Holbert S;Grépinet O;Velge P;Wiedemann A
通讯作者：
Wiedemann A
开放式细菌种群基因组学：BIGSDB软件，pubmlst.org网站及其应用。
影响因子：
--
作者：
Jolley KA;Bray JE;Maiden MCJ
通讯作者：
Maiden MCJ
影响因子：
5.2
作者：
Alshalchi S;Hayer SS;An R;Munoz-Aguayo J;Flores-Figueroa C;Nguyen R;Lauer D;Olsen K;Alvarez J;Boxrud D;Cardona C;Vidovic S
通讯作者：
Vidovic S
影响因子：
5.7
作者：
Liebl D;Qi X;Zhe Y;Barnett TC;Teasdale RD
通讯作者：
Teasdale RD
DOI：
10.1073/pnas.1822018116
发表时间：
2019-06-18
影响因子：
11.1
作者：
Desai, Stuti K.;Padmanabhan, Anup;Kenney, Linda J.
通讯作者：
Kenney, Linda J.