Fluorescent amplified fragment length polymorphism (AFLP) analysis of genetic diversity and relationship of Chinese Rosa rugosa germplasm resources
Fluorescent amplified fragment length polymorphism (AFLP) analysis of genetic diversity and relationship of Chinese Rosa rugosa germplasm resources
复制标题
荧光扩增片段长度多态性（AFLP）分析中国玫瑰种质资源遗传多样性及亲缘关系
DOI：
10.5897/ajb10.1294
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发表时间：
2011-05
影响因子：
--
通讯作者：
赵兰勇
中科院分区：
文献类型：
--
作者：
冯立国;生利霞;邵大伟;陶俊;赵兰勇
玫瑰是中国的优良观赏植物，具有重要的生态、经济和药用价值。利用荧光扩增片段长度多态性分子标记技术，对来自中国的5份野生玫瑰种质和25份栽培品种的基因组DNA进行了多态性分析，以评价它们的遗传多样性和亲缘关系。10对引物共扩增出1771条清晰可辨的条带(60~500bp)，其中1704条为多态条带。平均多态座位百分率为96.2%，表明中国玫瑰种质资源具有较高的遗传多样性。用10对引物从24份材料中扩增出不同数量的特异性条带，可用于鉴定玫瑰属植物的特异性。30份中国玫瑰种质资源的相似系数在0.4977~0.8410之间，平均为0.624，具有较高的相似性。聚类结果表明，来自中国不同地区的野生种质与丰富的遗传多样性有较远的亲缘关系。野生种质与栽培品种也有较远的亲缘关系。然而，我国主要栽培品种具有较高的相似性、较低的遗传差异和较窄的遗传背景。关键词：玫瑰，遗传多样性及亲缘关系，扩增片段长度多态性。
Rosa rugosa is an excellent ornamental plant with important ecological, economical and medicinal values in China. Polymorphism amplifications of the genomic DNA of 5 wild R. rugosa accessions and 25 cultivars that originated from China with fluorescent amplified fragment length polymorphism molecular markers were investigated in this study to evaluate their genetic diversity and relationships. A total of 1771 clearly identifiable bands (60 ~ 500 bp), including 1704 polymorphic bands, were obtained through amplification using 10 pairs of primers. The average percentage of polymorphic locus was 96.2%, which indicated the high genetic diversity of Chinese R. rugosa germplasm resources. Different quantities of specific bands were detected by 10 pairs of primers from 24 accessions, which could be used to identify the specific R. rugosa accession. There was high similarity between Chinese R. rugosa germplasm resources, as indicated by the similarity coefficient of 30 R. rugosa accessions between 0.4977 and 0.8410, with an average of 0.624. Clustering results suggested that wild accessions from different areas of China were remotely related with the ample genetic diversity. Wild accessions and cultivars also had remote genetic relationship. However, the main Chinese R. rugosa cultivars had high similarity, low genetic differences and a narrow genetic background. Key words : Rosa rugosa, genetic diversity and relationship, amplified fragment length polymorphism.
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影响因子：
1.9
作者：
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通讯作者：
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通讯作者：
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影响因子：
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