Mechanisms behind the inhibition of lung adenocarcinoma cell by shikonin.
Mechanisms behind the inhibition of lung adenocarcinoma cell by shikonin.
复制标题
紫草素抑制肺腺癌细胞的机制。
DOI：
10.1007/s12013-014-0083-5
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发表时间：
2014-11
影响因子：
2.6
通讯作者：
Zhou S
中科院分区：
文献类型：
--
作者：
Lan W;Wan S;Gu W;Wang H;Zhou S
紫草素是一种从传统中草药中分离得到的天然萘醌类化合物，具有抑制肿瘤细胞生长的作用。然而，紫草素对肺腺癌细胞的作用及其机制尚不清楚。本研究探讨紫草素对人肺腺癌细胞增殖、细胞周期阻滞和凋亡的影响。结果表明，紫草素对肺腺癌细胞的增殖有明显的抑制作用，且呈剂量和时间依赖性（P <0.05）。同时，我们的研究结果表明，紫草素显着增加A549细胞G1期的比例，并诱导A549细胞凋亡。此外，紫草素抑制肺腺癌细胞CCND1的表达，上调caspase 3和caspase 7的mRNA表达，提示紫草素可能通过调控CCND1、caspase 3和caspase 7的表达，改变细胞周期，促进细胞凋亡，从而抑制肺腺癌细胞的生长。虽然还需要更多的研究，但本研究表明紫草素具有作为治疗肺腺癌的抗癌药物的潜力。
Shikonin, a natural naphthoquinone isolated from a traditional Chinese medicinal herb, can exert inhibitory effect on tumor cell growth. However, little has been known concerning the effect of shikonin on lung adenocarcinoma cell and underlying mechanisms. In the present study, we investigated the effect of shikonin on the proliferation, cell cycle arrest, and apoptosis in human lung adenocarcinoma cells. We found that shikonin significantly suppressed the proliferation of lung adenocarcinoma cells compared with control in dose- and time-dependent manner (P < 0.05). In the meantime, our results showed that shikonin markedly increased the proportion of A549 cells at stage G1 as well as induced apoptosis in A549 cells. Furthermore, suppressed CCND1 and elevated caspase3 and caspase7 expression levels at mRNA were found in this study, indicating that shikonin may inhibit the growth of lung adenocarcinoma cell by changing cell cycle and promoting cell apoptosis through the regulation of CCND1, caspase3, and caspase7. Although more studies are needed, this study suggests that shikonin has the potential to be used as an anti-cancer agent in the treatment of lung adenocarcinoma.
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影响因子：
158.5
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