Functional interaction between RNase III and the Escherichia coli ribosome.
Functional interaction between RNase III and the Escherichia coli ribosome.
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DOI：
10.1186/1471-2199-4-8
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发表时间：
2003-06-18
影响因子：
--
通讯作者：
Remme J
中科院分区：
文献类型：
--
作者：
Allas U;Liiv A;Remme J
RNase III is a dsRNA specific endoribonuclease which is involved in the primary processing of rRNA and several mRNA species in bacteria. Both primary structural elements and the secondary structure of the substrate RNA play a role in cleavage specificity. We have analyzed RNase III cleavage sites around both ends of pre-23 S rRNA in the ribosome and in the protein-free pre-rRNA. It was found that in the protein-free pre-23 S rRNA the main cleavage site is at position (-7) in respect of the mature 5' end. When pre-23 S rRNA was in 70 S ribosomes or in 50 S subunits, the RNase III cleavage occurred at position (-3). We have demonstrated that RNase III interacts with both ribosomal subunits and with even higher affinity with 70 S ribosomes. Association of RNase III with 70 S ribosomes cannot be dissociated by poly(U) RNA indicating that the binding is specific. In addition to the primary and secondary structural elements in RNA, protein binding to substrate RNA can be a determinant of the RNase III cleavage site.
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影响因子：
11.4
作者：
KHARRAT, A;MACIAS, MJ;PASTORE, A
通讯作者：
PASTORE, A
影响因子：
14.9
作者：
Gregory, ST;OConnor, M;Dahlberg, AE
通讯作者：
Dahlberg, AE
DOI：
10.1007/bf00330746
发表时间：
1985-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
NASHIMOTO, H;MIURA, A;UCHIDA, H
通讯作者：
UCHIDA, H
影响因子：
4.8
作者：
Wu, HJ;Xu, H;Crooke, ST
通讯作者：
Crooke, ST
影响因子：
5.6
作者：
LEWICKI, BTU;MARGUS, T;NIERHAUS, KH
通讯作者：
NIERHAUS, KH