Analysis of Genes and Proteins in Acidithiobacillus ferrooxidans During Growth and Attachment on Pyrite Under Different Conditions
Analysis of Genes and Proteins in Acidithiobacillus ferrooxidans During Growth and Attachment on Pyrite Under Different Conditions
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不同条件下氧化亚铁硫杆菌生长及附着黄铁矿过程中的基因和蛋白质分析
DOI：
10.1080/01490451.2012.668608
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发表时间：
2013-01
影响因子：
2.3
通讯作者：
Xiaodong Han
中科院分区：
文献类型：
--
作者：
Bowen Tu;Fuqiang Wang;Juan Li;Jishao Sha;Xiancai Lu;Xiaodong Han
微生物能够促进硫化物矿物的分解，进而导致酸性矿山排水（AMD）的形成。细菌细胞附着在矿物表面是氧化亚铁硫杆菌氧化黄铁矿的一个重要过程。这种选择性优势 （你提供的内容“the selective advantag”似乎不完整，少了字母“e”，完整的“advantage”意思是“优势” ）
Microbes are able to enhance the sulfide mineral decomposition, which lead to the formation of AMD. Attachment of bacterial cells to the mineral surface is an important process for pyrite oxidation by Acidithiobacillus ferrooxidans. The selective advantage of bacterial adhesion is considered to favor the surface localization of bacterial populations as nutritionally favorable. Environmental factors determine cell accumulation or dissociation of attachment. In our study, the amount of sessile cells increased rapidly during the initial stage of attachment on pyrite. Planktonic cells showed high activity leading to the accumulation of large colonies on the pyrite surface. We found three proteins to be up-regulated significantly. Additionally, by matching the sequences of the three proteins to the Pfam database, we found that they are related to adhesion, pili biosynthesis and movement. When we replaced pyrite with glass to provide an inert surface that abolished electrostatic forces, we found that cell attachment was maintained under nutrient-rich conditions but drastically reduced under conditions of limited nutrients or the presence of the inhibitor homoserinelactone. Our results are consistent with the idea that starvation may lead to inhibition of attachment by an unknown mechanism that allows bacteria to search for nutrient-rich habitats.
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影响因子：
4.4
作者：
Valdes, Jorge;Pedroso, Inti;Quatrini, Raquel;Dodson, Robert J.;Tettelin, Herve;Blake, Robert, II;Eisen, Jonathan A.;Holmes, David S.
通讯作者：
Holmes, David S.
影响因子：
2.1
作者：
D. Allison;Begoña Ruiz;C. Sanjosé;A. Jaspe;P. Gilbert
通讯作者：
D. Allison;Begoña Ruiz;C. Sanjosé;A. Jaspe;P. Gilbert
影响因子：
4
作者：
Béchet, M;Blondeau, R
通讯作者：
Blondeau, R
影响因子：
3.1
作者：
Cramton, SE;Gerke, C;Götz, F
通讯作者：
Götz, F
影响因子：
3.1
作者：
MACK, D;NEDELMANN, M;LAUFS, R
通讯作者：
LAUFS, R