Dynamic succession of soil bacterial community during continuous cropping of peanut (Arachis hypogaea L.).
Dynamic succession of soil bacterial community during continuous cropping of peanut (Arachis hypogaea L.).
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花生连作过程中土壤细菌群落的动态演替（ArachishypogaeaL.）
DOI：
10.1371/journal.pone.0101355
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yu S
中科院分区：
文献类型：
--
作者：
Chen M;Li X;Yang Q;Chi X;Pan L;Chen N;Yang Z;Wang T;Wang M;Yu S
植物健康和土壤肥力受到土壤中植物-微生物相互作用的影响。花生是世界上重要的油料作物，具有很强的适应性，但连作对花生的生长发育和产量产生不利影响。本研究利用16 S rRNA基因克隆文库分析方法研究了花生连作土壤细菌群落的演替规律。以花生为研究对象，构建了3个连作周期、苗期和荚果成熟期的6个文库。聚类分析表明，从同一种植周期获得的土壤细菌组合相似，无论生育期。3个花生种植周期各生育期文库中鉴定的细菌序列多样性较高，分属于21个细菌类群。八门：优势菌为酸性细菌、放线菌、拟杆菌、绿弯菌、芽单胞菌、浮游菌、变形菌和疣微菌。花生连作过程中相关细菌类群的动态变化。本研究表明，细菌种群，特别是有益种群的积极选择。连作条件下，交替单胞菌目、伯克氏菌目、黄杆菌目、假单胞菌目、根瘤菌目和红球藻目等优势菌群的单一化可能是导致花生产量下降的重要因素。在连作过程中没有发生连续变化的微生物类群，如根瘤菌目和杜鹃菌目，可能具有抵抗连作胁迫的潜力，值得关注。此外，一些优势类群如酸杆菌目、染色菌目和芽单胞菌目的丰度或多样性则表现出相反的趋势，随着花生连作的进行，其丰度或多样性增加。一些细菌类群如酸杆菌目、伯克氏菌目、蛭弧菌目等也受到树龄的影响。
Plant health and soil fertility are affected by plant–microbial interactions in soils. Peanut is an important oil crop worldwide and shows considerable adaptability, but growth and yield are negatively affected by continuous cropping. In this study, 16S rRNA gene clone library analyses were used to study the succession of soil bacterial communities under continuous peanut cultivation. Six libraries were constructed for peanut over three continuous cropping cycles and during its seedling and pod-maturing growth stages. Cluster analyses indicated that soil bacterial assemblages obtained from the same peanut cropping cycle were similar, regardless of growth period. The diversity of bacterial sequences identified in each growth stage library of the three peanut cropping cycles was high and these sequences were affiliated with 21 bacterial groups. Eight phyla: Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia were dominant. The related bacterial phylotypes dynamic changed during continuous cropping progress of peanut. This study demonstrated that the bacterial populations especially the beneficial populations were positively selected. The simplification of the beneficial microbial communities such as the phylotypes of Alteromonadales, Burkholderiales, Flavobacteriales, Pseudomonadales, Rhizobiales and Rhodospirillales could be important factors contributing to the decline in peanut yield under continuous cropping. The microbial phylotypes that did not successively changed with continuous cropping, such as populations related to Rhizobiales and Rhodospirillales, could potentially resist stress due to continuous cropping and deserve attention. In addition, some phylotypes, such as Acidobacteriales, Chromatiales and Gemmatimonadales, showed a contrary tendency, their abundance or diversity increased with continuous peanut cropping progress. Some bacterial phylotypes including Acidobacteriales, Burkholderiales, Bdellovibrionales, and so on, also were affected by plant age.
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影响因子：
9.7
作者：
Almario, Juliana;Moenne-Loccoz, Yvan;Muller, Daniel
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.8
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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