Microsatellite DNA markers associated with resistance to WSSV in Penaeus (Fenneropenaeus) chinensis
Microsatellite DNA markers associated with resistance to WSSV in Penaeus (Fenneropenaeus) chinensis
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与中国对虾 (Fenneropenaeus) WSSV 抗性相关的微卫星 DNA 标记
DOI：
10.1016/j.aquaculture.2008.05.037
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发表时间：
2008-09
期刊：
影响因子：
4.5
通讯作者：
Wang, Rucai
中科院分区：
文献类型：
--
作者：
Dong, Shirui;Meng, Xianhong;Zhang, Qingwen;Kong, Jie;Zhang, Tianshi;Wang, Rucai
以f2科中国对虾（Fenneropenaeus chinensis）为研究对象，分析了微卫星DNA标记与中国对虾对白斑综合征病毒（WSSV）抗性的关系。在室内池中对f2家族163例个体进行口腔感染攻毒。采用巢式pcr检测WSSV的感染情况。选取18对引物对f2家族80个个体进行基因分型。用方差分析分析单个标记等位基因与WSSV抗性的关系。结果表明，所有攻毒对虾均被证实感染了WSSV。平均等位基因数为2.50，平均期望杂合度为0.536，平均多态信息含量为0.446。微卫星位点RS0622上的一个等位基因与WSSV的抗性相关。鉴定出更多与WSSV抗性QTL相关的DNA标记，将有助于DNA标记辅助选择技术在中国对虾水产养殖育种中的应用。
An F2family of Chinese shrimp, Penaeus (Fenneropenaeus) chinensis, was used to conduct analysis of an association between microsatellite DNA markers and resistance to WSSV (white spot syndrome virus). The total of 163 individuals from the F2family was challenged with WSSV by oral infection in an indoor tank. Nested-PCR was used to test for infection of WSSV. Eighteen primer pairs were selected to genotype 80 individuals in the F2family. Associations of individual marker alleles with resistance to WSSV were analysed by ANOVA. The results showed that all shrimp challenged were confirmed to have been infected by WSSV. The mean number of alleles, mean expected heterozygosity, and mean polymorphic information content were 2.50, 0.536 and 0.446, respectively. An allele at microsatellite locus RS0622 was associated with resistance to WSSV. Identification of a larger number of DNA markers linked to QTL controlling the resistance to WSSV will contribute to the application of DNA marker-assisted selection in aquacultural breeding of Chinese shrimp.
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影响因子：
1.4
作者：
Li Shuijun;Ma Jiahai;Ji Huanhong;Xie Enyi
通讯作者：
Li Shuijun;Ma Jiahai;Ji Huanhong;Xie Enyi
影响因子：
3.1
作者：
Robison, BD;Wheeler, PA;Thorgaard, GH
通讯作者：
Thorgaard, GH
影响因子：
2.4
作者：
E. Hallerman;J. Beckmann
通讯作者：
E. Hallerman;J. Beckmann
影响因子：
4.5
作者：
T. Sakamoto;R. Danzmann;N. Okamoto;M. Ferguson;P. Ihssen
通讯作者：
T. Sakamoto;R. Danzmann;N. Okamoto;M. Ferguson;P. Ihssen
影响因子：
14.9
作者：
LOVE, JM;KNIGHT, AM;TODD, JA
通讯作者：
TODD, JA