Diversity and antioxidant activity of culturable endophytic fungi from alpine plants of Rhodiola crenulata, R. angusta, and R. sachalinensis.
Diversity and antioxidant activity of culturable endophytic fungi from alpine plants of Rhodiola crenulata, R. angusta, and R. sachalinensis.
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DOI：
10.1371/journal.pone.0118204
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wang ML
中科院分区：
文献类型：
--
作者：
Cui JL;Guo TT;Ren ZX;Zhang NS;Wang ML
红景天属是珍稀濒危高山植物，自古以来被许多文明广泛用作药物和食品添加剂。其主要有效成分（如红景天苷和对酪醇）因其抗氧化特性而被誉为对高原反应具有药理作用，并具有抗衰老和其他适应能力。本研究从R. crenulata、R. angusta和R. sachalinensis中分离得到347株内生真菌，并首次研究了这些真菌的分子多样性和抗氧化活性。根据培养特征将这些真菌分为180个形态型，并在GenBank数据库中通过BLAST搜索对其rRNA基因ITS序列进行分析。除12种未鉴定真菌（6.67%）外，其余真菌均隶属于子囊菌门（88.89%）、担子菌门（2.78%）、接合菌门（1.11%）和球囊菌门（0.56%）4门20目至少57属，表现出较高的丰度和多样性。抗氧化检测结果显示，114株菌株（63.33%）的DPPH自由基清除率>50%，其中5株（Rct45、Rct63、Rct64、Rac76、Rsc57）的DPPH自由基清除率>90%。五项抗氧化剂测定的 EC50 值表明这些真菌在清除 DPPH•、O2−• 和 OH• 自由基以及清除亚硝酸盐和螯合 Fe2+ 方面具有显着潜力，这表明内生真菌与其宿主之间存在偏好和选择。进一步的研究还首次提供了 Rac12 可以产生红景天苷和对酪醇的证据。结果表明，与红景天相关的多功能内生真菌（称为抗氧化剂）可以作为新型抗氧化剂产品的潜在来源。
Rhodiola spp. are rare and endangered alpine plants widely used as medicines and food additives by many civilizations since ancient times. Their main effective ingredients (such as salidroside and p-tyrosol) are praised to exhibit pharmacologic effects on high-altitude sickness and possess anti-aging and other adaptogenic capacities based on their antioxidant properties. In this study, 347 endophytic fungi were isolated from R. crenulata, R. angusta, and R. sachalinensis, and the molecular diversity and antioxidant activities of these fungi were investigated for the first time. These fungi were categorized into 180 morphotypes based on cultural characteristics, and their rRNA gene ITS sequences were analyzed by BLAST search in the GenBank database. Except for 12 unidentified fungi (6.67%), all others were affiliated to at least 57 genera in 20 orders of four phyla, namely, Ascomycota (88.89%), Basidiomycota (2.78%), Zygomycota (1.11%), and Glomeromycota (0.56%), which exhibited high abundance and diversity. Antioxidant assay showed that the DPPH radical-scavenging rates of 114 isolates (63.33%) were >50%, and those of five isolates (Rct45, Rct63, Rct64, Rac76, and Rsc57) were >90%. The EC50 values of five antioxidant assays suggested significant potential of these fungi on scavenging DPPH•, O2−•, and OH• radicals, as well as scavenging nitrite and chelating Fe2+, which showed preference and selection between endophytic fungi and their hosts. Further research also provided the first evidence that Rac12 could produce salidrosides and p-tyrosol. Results suggested that versatile endophytic fungi associated with Rhodiola known as antioxidants could be exploited as potential sources of novel antioxidant products.
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影响因子：
2.8
作者：
Arnold, A. Elizabeth;Henk, Daniel A.;Vilgalys, Rvtas
通讯作者：
Vilgalys, Rvtas
DOI：
10.1073/pnas.0401513101
发表时间：
2004-07-06
影响因子：
11.1
作者：
Ganley, RJ;Brunsfeld, SJ;Newcombe, G
通讯作者：
Newcombe, G
DOI：
10.1002/nt.2620010306
发表时间：
1992-01-01
期刊：
Natural Toxins
影响因子：
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作者：
Petrini, Orlando;Sieber, Thomas N.;Viret, Olivier
通讯作者：
Viret, Olivier
影响因子：
4.1
作者：
Nicoletti, Rosario;Buommino, Elisabetta;Tufano, Maria Antonietta
通讯作者：
Tufano, Maria Antonietta
影响因子：
6.1
作者：
Chu, Yung-Hung;Chen, Chao-Jung;Hsieh, Jung-Feng
通讯作者：
Hsieh, Jung-Feng