Sex differences in global mRNA content of human skeletal muscle.
Sex differences in global mRNA content of human skeletal muscle.
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DOI：
10.1371/journal.pone.0006335
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发表时间：
2009-07-22
期刊：
影响因子：
3.7
通讯作者：
Tarnopolsky MA
中科院分区：
文献类型：
--
作者：
Maher AC;Fu MH;Isfort RJ;Varbanov AR;Qu XA;Tarnopolsky MA
在耐力运动期间，与男性相比，女性氧化更多的脂肪，并且几个研究小组已经表明，女性中与脂肪氧化相关的选定基因的mRNA含量较高（例如激素敏感性脂肪酶，β-羟酰辅酶A脱氢酶，CD 36）。其中一个可能的机制是，与男性相比，女性往往具有更高的I型骨骼肌纤维面积百分比。因此，我们假设性别会影响参与代谢和肌纤维类型决定的基因的基础mRNA和蛋白质含量。从健康男性和女性的股外侧肌收集肌肉活检。我们使用Affyssin GeneChips全面检查mRNA含量，并通过RT-PCR检查和/或确认选定的基因。此外，我们通过Western印迹分析检测蛋白质含量。严格的基因阵列分析显示66个差异表达基因，分别代表代谢、线粒体功能、转运、蛋白质生物合成、细胞增殖、信号转导途径、转录和翻译。严格基因阵列分析和RT-PCR证实，酰基辅酶A酰基转移酶2（ACAA 2）、三功能蛋白β（HADHB）、过氧化氢酶、脂蛋白脂酶（LPL）和解偶联蛋白-2（UCP-2）的mRNA在女性中较高。靶基因分析显示，女性肌球蛋白重链I（MHCI）、过氧化物酶体增殖物激活受体（PPAR）δ的表达高于男性。令人惊讶的是，HADHB、ACAA 2、过氧化氢酶、PPARδ和MHC 1的蛋白质含量没有显著的性别差异。总之，静息骨骼肌中基础mRNA含量的差异表明，男性和女性在转录上“启动”了已知的代谢生理差异，但纤维类型性别差异背后的机制仍有待确定。
Women oxidize more fat as compared to men during endurance exercise and several groups have shown that the mRNA content of selected genes related to fat oxidation are higher in women (e.g. hormone sensitive lipase, β-hydroxyacyl-CoA dehydrogenase, CD36). One of the possible mechanisms is that women tend to have a higher area percentage of type I skeletal muscle fibers as compared with men. Consequently, we hypothesized that sex would influence the basal mRNA and protein content for genes involved in metabolism and the determination of muscle fiber type. Muscle biopsies from the vastus lateralis were collected from healthy men and women. We examined mRNA content globally using Affymetrix GeneChips, and selected genes were examined and/or confirmed by RT-PCR. Furthermore, we examined protein content by Western blot analysis. Stringent gene array analysis revealed 66 differentially expressed genes representing metabolism, mitochondrial function, transport, protein biosynthesis, cell proliferation, signal transduction pathways, transcription and translation. Stringent gene array analysis and RT-PCR confirmed that mRNA for; acyl-coenzyme A acyltransferase 2 (ACAA2), trifunctional protein β (HADHB), catalase, lipoprotein lipase (LPL), and uncoupling protein-2 (UCP-2) were higher in women. Targeted gene analysis revealed that myosin heavy chain I (MHCI), peroxisome proliferator-activated receptor (PPAR)δ were higher in women compared with men. Surprisingly, there were no significant sex based differences in protein content for HADHB, ACAA2, catalase, PPARδ, and MHC1. In conclusion, the differences in the basal mRNA content in resting skeletal muscle suggest that men and women are transcriptionally “primed” for known physiological differences in metabolism however the mechanism behind sex differences in fiber type remains to be determined.
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影响因子：
15.9
作者：
Djouadi, F;Weinheimer, CJ;Kelly, DP
通讯作者：
Kelly, DP
DOI：
10.1073/pnas.95.25.14938
发表时间：
1998-12-08
影响因子：
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作者：
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作者：
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