Transcriptome Analysis of Green Peach Aphid (Myzus persicae): Insight into Developmental Regulation and Inter-Species Divergence.
Transcriptome Analysis of Green Peach Aphid (Myzus persicae): Insight into Developmental Regulation and Inter-Species Divergence.
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绿桃蚜（Myzus persicae）的转录组分析：深入了解发育调控和种间差异。
DOI：
10.3389/fpls.2016.01562
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发表时间：
2016
影响因子：
5.6
通讯作者：
Zhu-Salzman K
中科院分区：
文献类型：
--
作者：
Ji R;Wang Y;Cheng Y;Zhang M;Zhang HB;Zhu L;Fang J;Zhu-Salzman K
桃蚜（Myzus persicae）和豌豆蚜（Acyrthosiphon pisum）是两种系统发育密切相关的农业害虫。虽然豌豆蚜仅限于豆科植物，但绿桃蚜却以 40 多个科的数百种植物为食。转录组比较可以揭示两个物种之间宿主范围差异背后的遗传因素。此外，比较未成熟若虫和完全发育的成年蚜虫之间基因表达的大规模研究将填补先前的知识空白。在这里，我们利用 Illumina 测序技术分别获得了桃蚜若虫和成虫的转录组序列。总共发现2244个基因在两个发育阶段之间存在差异表达，其中许多与解毒、激素产生、角质层形成、新陈代谢、食物消化和吸收有关。当对公开的豌豆蚜 mRNA 序列进行搜索时，发现 13,752 个 unigene 没有同源对应物。有趣的是，许多可以在其他数据库中注释的unigenes都涉及“外源生物降解和代谢”途径，这表明这两种蚜虫在对寄主植物次生代谢物的适应方面存在差异。相反，两个物种之间的 3989 个直系同源基因对进行了同义和非同义替换的计算，其中 148 个基因可能响应正选择而进化。其中一些基因预计与昆虫与植物的相互作用有关。我们的研究揭示了与蚜虫发育相关的某些分子事件，并提供了对两种蚜虫物种的生物变异的一些见解，这些变异可能是寄主植物适应的结果。
Green peach aphid (Myzus persicae) and pea aphid (Acyrthosiphon pisum) are two phylogenetically closely related agricultural pests. While pea aphid is restricted to Fabaceae, green peach aphid feeds on hundreds of plant species from more than 40 families. Transcriptome comparison could shed light on the genetic factors underlying the difference in host range between the two species. Furthermore, a large scale study contrasting gene expression between immature nymphs and fully developed adult aphids would fill a previous knowledge gap. Here, we obtained transcriptomic sequences of green peach aphid nymphs and adults, respectively, using Illumina sequencing technology. A total of 2244 genes were found to be differentially expressed between the two developmental stages, many of which were associated with detoxification, hormone production, cuticle formation, metabolism, food digestion, and absorption. When searched against publically available pea aphid mRNA sequences, 13,752 unigenes were found to have no homologous counterparts. Interestingly, many of these unigenes that could be annotated in other databases were involved in the “xenobiotics biodegradation and metabolism” pathway, suggesting the two aphids differ in their adaptation to secondary metabolites of host plants. Conversely, 3989 orthologous gene pairs between the two species were subjected to calculations of synonymous and nonsynonymous substitutions, and 148 of the genes potentially evolved in response to positive selection. Some of these genes were predicted to be associated with insect-plant interactions. Our study has revealed certain molecular events related to aphid development, and provided some insight into biological variations in two aphid species, possibly as a result of host plant adaptation.
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影响因子：
4.4
作者：
Bansal R;Mian MA;Mittapalli O;Michel AP
通讯作者：
Michel AP
DOI：
10.1094/mpmi-01-14-0018-r
发表时间：
2014-07
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Elzinga DA;De Vos M;Jander G
通讯作者：
Jander G
影响因子：
2.2
作者：
FELTON, GW;SUMMERS, CB
通讯作者：
SUMMERS, CB
影响因子：
4.6
作者：
He Q;Jones DC;Li W;Xie F;Ma J;Sun R;Wang Q;Zhu S;Zhang B
通讯作者：
Zhang B
影响因子：
2.6
作者：
BANKS, CJ;MACAULAY, ED
通讯作者：
MACAULAY, ED