The pathway to detangle a scrambled gene.
The pathway to detangle a scrambled gene.
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DOI：
10.1371/journal.pone.0002330
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发表时间：
2008-06-04
期刊：
影响因子：
3.7
通讯作者：
Landweber LF
中科院分区：
文献类型：
--
作者：
Möllenbeck M;Zhou Y;Cavalcanti AR;Jönsson F;Higgins BP;Chang WJ;Juranek S;Doak TG;Rozenberg G;Lipps HJ;Landweber LF
程序性DNA消除和重组经常发生在细胞分化过程中。一些纤毛虫体细胞大核的发育是一个极端的情况，包括切除中断编码DNA片段（大核目的序列，MDS）的内部消除序列（IES），以及去除转座子样元件和广泛的基因组片段，导致98%的Stylonychia lemorrhage基因组减少。据估计，种系微核中大约20-30%的基因被扰乱，编码片段顺序发生排列，并以任一方向存在于微核染色体上。因此，大规模基因组重排对发展至关重要。为了了解大核发育过程中DNA缺失和重组的过程，我们通过PCR检测了两种相关生物在发育时间过程中不同乱序基因组装过程中的DNA分子群体。数据表明，通常首先删除传统的IES，在这一步伴随着惊人的错误水平。倒置和易位的复杂事件似乎发生在所有常规IEs修复和切除后，并通过多种途径。 这项研究揭示了一个时间顺序的DNA重排过程中的一个乱序基因，与简单的事件通常之前更复杂的。令人惊讶的观察到一个隐藏的错误层，不存在于成熟的大核中，但在发育过程中存在，这也强调了修复或筛选错误组装的DNA分子的必要性。
Programmed DNA elimination and reorganization frequently occur during cellular differentiation. Development of the somatic macronucleus in some ciliates presents an extreme case, involving excision of internal eliminated sequences (IESs) that interrupt coding DNA segments (macronuclear destined sequences, MDSs), as well as removal of transposon-like elements and extensive genome fragmentation, leading to 98% genome reduction in Stylonychia lemnae. Approximately 20–30% of the genes are estimated to be scrambled in the germline micronucleus, with coding segment order permuted and present in either orientation on micronuclear chromosomes. Massive genome rearrangements are therefore critical for development. To understand the process of DNA deletion and reorganization during macronuclear development, we examined the population of DNA molecules during assembly of different scrambled genes in two related organisms in a developmental time-course by PCR. The data suggest that removal of conventional IESs usually occurs first, accompanied by a surprising level of error at this step. The complex events of inversion and translocation seem to occur after repair and excision of all conventional IESs and via multiple pathways. This study reveals a temporal order of DNA rearrangements during the processing of a scrambled gene, with simpler events usually preceding more complex ones. The surprising observation of a hidden layer of errors, absent from the mature macronucleus but present during development, also underscores the need for repair or screening of incorrectly-assembled DNA molecules.
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影响因子：
14.9
作者：
Cavalcanti, ARO;Clarke, TH;Landweber, LF
通讯作者：
Landweber, LF
DOI：
10.1016/s0167-5699(05)80009-x
发表时间：
1991-03-01
期刊：
IMMUNOPARASITOLOGY TODAY-A COMBINED ISSUE OF IMMUNOLOGY TODAY AND PARASITOLOGY TODAY
影响因子：
--
作者：
BORST, P
通讯作者：
BORST, P
影响因子：
10.5
作者：
Chalker, DL;Yao, MC
通讯作者：
Yao, MC
DNA超螺旋抑制了实时PCR：一种定量线粒体DNA损伤和修复的新方法。
影响因子：
14.9
作者：
Chen J;Kadlubar FF;Chen JZ
通讯作者：
Chen JZ
影响因子：
5.8
作者：
Cavalcanti, ARO;Landweber, LF
通讯作者：
Landweber, LF