The role of hypertriglyceridemia in the development of atherosclerosis and endothelial dysfunction.
The role of hypertriglyceridemia in the development of atherosclerosis and endothelial dysfunction.
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DOI：
10.3390/nu6031236
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发表时间：
2014-03-24
期刊：
影响因子：
5.9
通讯作者：
Ishihata A
中科院分区：
文献类型：
--
作者：
Matsumoto S;Gotoh N;Hishinuma S;Abe Y;Shimizu Y;Katano Y;Ishihata A
遗传性餐后高脂血症兔（PHT兔）是一种新的血脂异常模型，表现为血浆甘油三酯显著升高，而血浆总胆固醇仅有限升高。在PHT家兔中，与健康日本白色（JW）家兔相比，餐后血浆甘油三酯显著升高。在生理学实验中，将胸主动脉的环形制备物悬浮在充满改良Krebs-Henseleit溶液的器官浴中，并记录所产生的张力。内皮功能评价乙酰胆碱诱导的血管舒张在每个制备与完整的内皮。PHT兔乙酰胆碱诱导的内皮依赖性舒张功能较JW兔减弱，提示PHT兔内皮功能障碍。在脂肪组织、肝脏和主动脉中进行组织学检查。将它们固定在甲醛中并包埋在石蜡中。将组织切片（4 μm）并使用苏木精-伊红溶液染色。在脂肪组织中，PHT兔内脏脂肪堆积，脂肪细胞体积增大。PHT兔肝脏脂肪变性。在主动脉中，观察到内膜厚度增加，表明PHT兔中动脉粥样硬化的进展。提示餐后高血脂在动脉粥样硬化中的重要作用。用PHT家兔可以准确评价高脂血症对健康和疾病的影响。
A hereditary postprandial hypertriglyceridemic rabbit (PHT rabbit) is a new dyslipidemic model showing remarkably high plasma triglycerides with only limited elevation of plasma total cholesterol. In PHT rabbits, plasma triglyceride was markedly elevated postprandially compared with healthy Japanese white (JW) rabbits. In physiological experiments, the ring preparation of the thoracic aorta was suspended in an organ bath filled with modified Krebs-Henseleit solution, and the developed tension was recorded. Endothelial function was evaluated by acetylcholine-induced vasorelaxation in each preparation with intact endothelium. The acetylcholine-induced endothelium-dependent relaxation was diminished in PHT compared with JW rabbits, suggesting endothelial dysfunction in PHT rabbits. Histological examination was carried out in adipose tissue, liver and aorta. They were fixed in formaldehyde and embedded in paraffin. The tissues were sliced (4 μm) and stained using hematoxylin-eosin solution. In the adipose tissue, the visceral fat accumulated, and the size of adipose cells was enlarged in PHT rabbits. The liver of the PHT rabbit was fatty and degenerated. In aorta, increased intimal thickness was observed, suggesting the progression of atherosclerosis in the PHT rabbit. This study suggests the important role of postprandial hypertriglyceridemia in atherosclerosis. By using PHT rabbits, the effects of hypertriglyceridemia on health and diseases could be evaluated precisely.
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影响因子：
2.4
作者：
Fukuda, Naoki;Ito, Tsunekata;Fujii, Junichi
通讯作者：
Fujii, Junichi
影响因子：
37.8
作者：
Boquist, S;Ruotolo, G;Hamsten, A
通讯作者：
Hamsten, A
影响因子：
37.8
作者：
Miller, Michael;Stone, Neil J.;Pennathur, Subramanian
通讯作者：
Pennathur, Subramanian
影响因子：
2.4
作者：
Hata, M.;Ito, T.;Ohwada, K.
通讯作者：
Ohwada, K.
DOI：
10.1111/j.1467-789x.2004.00126.x
发表时间：
2004-02-01
期刊：
Obesity reviews : an official journal of the International Association for the Study of Obesity
影响因子：
--
作者：
Festi, D;Colecchia, A;Marchesini, G
通讯作者：
Marchesini, G