Capillary electrophoresis of complex natural polysaccharides.
Capillary electrophoresis of complex natural polysaccharides.
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DOI：
10.1002/elps.200800109
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发表时间：
2008-08
期刊：
影响因子：
2.9
通讯作者：
Linhardt, Robert J.
中科院分区：
文献类型：
--
作者：
Volpi, Nicola;Maccari, Francesca;Linhardt, Robert J.
糖胺多糖(GAG)是一类广泛存在的大分子物质，具有广泛的生物学功能，参与和调节多种细胞事件和(病理)生理过程。它们通常以自由链(透明质酸和细菌酸性多糖)或蛋白多糖的侧链(PGs；软骨素/皮肤素硫酸盐、肝素/肝素硫酸盐和角蛋白硫酸盐)存在，最常存在于细胞膜和细胞外基质中。最近出现的用于他们研究的现代分析工具在糖组学领域产生了实际上的爆炸性进展。毛细管电泳法具有较高的分辨率和灵敏度，可用于分析完整的GAG和GAG衍生的寡糖和二糖，为理解GAG的生物学功能提供必要的浓度和结构表征数据。本文综述了分离和在线CE-MS和MS/MS新方法在GAG低聚糖和双糖筛选中的应用。
Complex natural polysaccharides, glycosaminoglycans (GAGs), are a class of ubiquitous macromolecules that exhibit a wide range of biological functions and participate and regulate multiple cellular events and (patho)physiological processes. They are generally present either as free chains (hyaluronic acid and bacterial acidic polysaccharides) or as side chains of proteoglycans (PGs; chondroitin/dermatan sulfate, heparin/heparan sulfate, and keratan sulfate) and are most often found in cell membranes and in the extracellular matrix. The recent emergence of modern analytical tools for their study has produced a virtual explosion in the field of glycomics. CE, due to its high resolving power and sensitivity, has been useful in the analysis of intact GAGs and GAG-derived oligosaccharides and disaccharides affording concentration and structural characterization data essential for understanding the biological functions of GAGs. In this review, novel off-line and on-line CE-MS and MS/MS methods for screening of GAG-derived oligosaccharides and disaccharides will be discussed.
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