Insecticide resistance status in Anopheles gambiae in southern Benin.
Insecticide resistance status in Anopheles gambiae in southern Benin.
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DOI：
10.1186/1475-2875-9-83
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发表时间：
2010-03-24
期刊：
影响因子：
3
通讯作者：
Akogbeto MC
中科院分区：
文献类型：
--
作者：
Yadouleton AW;Padonou G;Asidi A;Moiroux N;Bio-Banganna S;Corbel V;N'guessan R;Gbenou D;Yacoubou I;Gazard K;Akogbeto MC
冈比亚按蚊出现拟除虫菊酯耐药性已成为影响今后疟疾控制工作成功的一个严重问题。在贝宁，国家疟疾控制规划最近计划扩大长效杀虫蚊帐和室内滞留喷洒，以预防疟疾。因此，监测安家鸡对杀虫剂的抗性水平和类型至关重要。特别是在贝宁南部，以前曾报道过驱虫蚊帐（ITNs）和IRS的效力下降。该议定书的依据是在贝宁南部选定的40个地区在旱季和雨季收集蚊子。根据世卫组织指南，对野外采集的成人进行生物测定，评估疟疾病媒对浸渍纸（氯菊酯0.75%、溴氰菊酯0.05%、滴滴涕4%和恶虫威0.1%）的敏感性。安内的物种。PCR检测冈比亚菌复合体、分子形态以及kdr和ace-1突变的存在。对氯菊酯和滴滴涕有较强的抗性。贝宁南部的冈比亚种群，但阿格兰丹丹除外，那里的蚊子对所有所测杀虫剂完全敏感（死亡率100%）。PCR结果显示存在两个亚种。冈比亚，即安。冈比亚疟蚊和黑按蚊，以疟蚊为主。冈比亚（98%）。An的M分子形式。冈比亚族在贝宁南部占主导地位（97%）。kdr突变在所有地区以不同的频率（1%至95%）被检测到，而Ace-1突变在非常低的频率（≤5%）被发现。本研究表明，我国对氯菊酯存在广泛的抗性。来自贝宁南部的冈比亚人，与以前在贝宁观察到的情况相比，kdr频率显著增加。在贝宁，所有人群中记录到的Ace-1发生频率较低，这对于使用苯虫威作为除虫菊酯类杀虫剂的替代杀虫剂进行室内滞留是令人鼓舞的。
The emergence of pyrethroid resistance in Anopheles gambiae has become a serious concern to the future success of malaria control. In Benin, the National Malaria Control Programme has recently planned to scaling up long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) for malaria prevention. It is, therefore, crucial to monitor the level and type of insecticide resistance in An. gambiae, particularly in southern Benin where reduced efficacy of insecticide-treated nets (ITNs) and IRS has previously been reported. The protocol was based on mosquito collection during both dry and rainy seasons across forty districts selected in southern Benin. Bioassay were performed on adults collected from the field to assess the susceptibility of malaria vectors to insecticide-impregnated papers (permethrin 0.75%, delthamethrin 0.05%, DDT 4%, and bendiocarb 0.1%) following WHOPES guidelines. The species within An. gambiae complex, molecular form and presence of kdr and ace-1 mutations were determined by PCR. Strong resistance to permethrin and DDT was found in An. gambiae populations from southern Benin, except in Aglangandan where mosquitoes were fully susceptible (mortality 100%) to all insecticides tested. PCR showed the presence of two sub-species of An. gambiae, namely An. gambiae s.s, and Anopheles melas, with a predominance for An. gambiae s.s (98%). The molecular M form of An. gambiae was predominant in southern Benin (97%). The kdr mutation was detected in all districts at various frequency (1% to 95%) whereas the Ace-1 mutation was found at a very low frequency (≤ 5%). This study showed a widespread resistance to permethrin in An. gambiae populations from southern Benin, with a significant increase of kdr frequency compared to what was observed previously in Benin. The low frequency of Ace-1 recorded in all populations is encouraging for the use of bendiocarb as an alternative insecticide to pyrethroids for IRS in Benin.
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影响因子：
3
作者：
Corbel V;Chandre F;Brengues C;Akogbéto M;Lardeux F;Hougard JM;Guillet P
通讯作者：
Guillet P
DOI：
10.1016/s0035-9203(99)90344-9
发表时间：
1999-09-01
影响因子：
2.2
作者：
Lindblade, KA;Walker, ED;Wilson, ML
通讯作者：
Wilson, ML
影响因子：
2.6
作者：
Fanello, C;Petrarca, V;Coluzzi, M
通讯作者：
Coluzzi, M
影响因子：
3
作者：
Czeher, Cyrille;Labbo, Rabiou;Duchemin, Jean-Bernard
通讯作者：
Duchemin, Jean-Bernard
影响因子：
2.7
作者：
MAGESA, SM;WILKES, TJ;CURTIS, CF
通讯作者：
CURTIS, CF