Imaging of kiss-and-run exocytosis of surface receptors in neuronal cultures.
Imaging of kiss-and-run exocytosis of surface receptors in neuronal cultures.
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DOI：
10.3389/fncel.2014.00363
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发表时间：
2014
影响因子：
5.3
通讯作者：
Yudowski GA
中科院分区：
文献类型：
--
作者：
Roman-Vendrell C;Chevalier M;Acevedo-Canabal AM;Delgado-Peraza F;Flores-Otero J;Yudowski GA
通过高度调控和复杂的运输步骤，跨膜蛋白不断地从内体隔间穿梭到神经细胞膜。这些事件涉及许多动态平衡和生理过程，如神经元生长、信号、学习和记忆等。我们先前已经证明，B2型肾上腺素能受体(B2AR)和含有GluR1的AMPA受体的胞吐作用是由两种不同类型的囊泡融合所介导的。一种快速类型的胞吐作用，其中受体在单个动力学步骤中被输送到质膜；以及一种持续模式，在将受体输送到细胞表面之前，受体在插入部位保持一段不同的时间。在这里，通过比较分离的海马体和纹状体培养中多种受体的胞吐作用，我们表明持续性事件是囊泡传递的一般机制。持续事件仅在体外10天后观察到，且持续事件的频率随钙离子载体A23187的使用和氯化钾引起的去极化而增加。最后，我们确定产生持续性事件的囊泡仍然留在质膜上，关闭并重新打开它们的融合孔，以连续释放货物，这一机制使人想起突触接吻和逃跑的机制。这些结果表明，跨膜受体到细胞表面的传递可以通过接吻即跑的胞吐作用来动态调节。
Transmembrane proteins are continuously shuttled from the endosomal compartment to the neuronal plasma membrane by highly regulated and complex trafficking steps. These events are involved in many homeostatic and physiological processes such as neuronal growth, signaling, learning and memory among others. We have previously shown that endosomal exocytosis of the B2 adrenergic receptor (B2AR) and the GluR1-containing AMPA receptor to the neuronal plasma membrane is mediated by two different types of vesicular fusion. A rapid type of exocytosis in which receptors are delivered to the plasma membrane in a single kinetic step, and a persistent mode in which receptors remain clustered at the insertion site for a variable period of time before delivery to the cell surface. Here, by comparing the exocytosis of multiple receptors in dissociated hippocampal and striatal cultures, we show that persistent events are a general mechanism of vesicular delivery. Persistent events were only observed after 10 days in vitro, and their frequency increased with use of the calcium ionophore A23187 and with depolarization induced by KCl. Finally, we determined that vesicles producing persistent events remain at the plasma membrane, closing and reopening their fusion pore for a consecutive release of cargo in a mechanism reminiscent of synaptic kiss-and-run. These results indicate that the delivery of transmembrane receptors to the cell surface can be dynamically regulated by kiss-and-run exocytosis.
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影响因子：
64.5
作者：
Puthenveedu MA;Lauffer B;Temkin P;Vistein R;Carlton P;Thorn K;Taunton J;Weiner OD;Parton RG;von Zastrow M
通讯作者：
von Zastrow M
影响因子：
3.5
作者：
Lasiecka, Zofia M.;Winckler, Bettina
通讯作者：
Winckler, Bettina
影响因子：
4.8
作者：
Roman-Vendrell, Cristina;Yu, Y. Joy;Yudowski, Guillermo Ariel
通讯作者：
Yudowski, Guillermo Ariel
影响因子：
25
作者：
Staal, RGW;Mosharov, EV;Sulzer, D
通讯作者：
Sulzer, D
影响因子：
64.5
作者：
Jaiswal JK;Rivera VM;Simon SM
通讯作者：
Simon SM