Comparative evaluation of six commercialized multiplex PCR kits for the diagnosis of respiratory infections.
Comparative evaluation of six commercialized multiplex PCR kits for the diagnosis of respiratory infections.
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DOI：
10.1371/journal.pone.0072174
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Pozzetto B
中科院分区：
文献类型：
--
作者：
Pillet S;Lardeux M;Dina J;Grattard F;Verhoeven P;Le Goff J;Vabret A;Pozzetto B
呼吸道感染的分子诊断可以使用不同的商业多重PCR试剂盒进行，这些试剂盒的性能先前已经单独与常规技术进行了比较。本研究比较了2011年法国市场上6种CE认证试剂盒的实用性和诊断性能，包括2种检测病毒和非典型细菌（来自Pathofinder和Seegene公司）和4种仅检测病毒（来自Abbott、Genomica、Qiagen和Seegene公司）。通过与用作金标准的市售双重PCR检测（Argene/bioMérieux）进行比较，计算每种多重技术各自的灵敏度、特异性、准确度和一致性。选择88份无病原体（n = 11）、单一感染（n = 33）或合并感染（n =44）的呼吸道标本，涵盖9种病毒或病毒群和3种非典型细菌。 使用NUCLISENS® easyMAG™仪器（bioMérieux）提取所有样品。病毒的总体灵敏度范围为56.25%至91.67%，细菌的两种检测均低于50%。总体特异性极佳（所有病原体>94%）。对于每个测试的试剂盒，与参考测试的总体一致性对于病毒是强的（kappa检验>0.60），对于细菌是中等的。浸提步骤后，实际操作时间从50 min到2 h 30不等，完整结果可在2 h 30到9 h内获得。测试试剂的光谱和用于显示PCR产物的技术以及实验室组织是选择试剂盒的决定性因素。
The molecular diagnosis of respiratory infection can be performed using different commercial multiplex-based PCR kits whose performances have been previously compared individually to those of conventional techniques. This study compared the practicability and the diagnostic performances of six CE-marked kits available in 2011 on the French market, including 2 detecting viruses and atypical bacteria (from Pathofinder and Seegene companies) and 4 detecting only viruses (from Abbott, Genomica, Qiagen and Seegene companies). The respective sensitivity, specificity, accuracy and agreement of each multiplex technique were calculated by comparison to commercial duplex PCR tests (Argene/bioMérieux) used as gold standard. Eighty-eight respiratory specimens with no pathogen (n = 11), single infections (n = 33) or co-infections (n = 44) were selected to cover 9 viruses or groups of viruses and 3 atypical bacteria. All samples were extracted using the NUCLISENS® easyMAG™ instrument (bioMérieux). The overall sensitivity ranged from 56.25% to 91.67% for viruses and was below 50% with both tests for bacteria. The overall specificity was excellent (>94% for all pathogens). For each tested kit, the overall agreement with the reference test was strong for viruses (kappa test >0.60) and moderate for bacteria. After the extraction step, the hands-on time varied from 50 min to 2h30 and the complete results were available in 2h30 to 9 h. The spectrum of tested agents and the technology used to reveal the PCR products as well as the laboratory organization are determinant for the selection of a kit.
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影响因子：
3.1
作者：
Bellau-Pujol S;Vabret A;Legrand L;Dina J;Gouarin S;Petitjean-Lecherbonnier J;Pozzetto B;Ginevra C;Freymuth F
通讯作者：
Freymuth F
影响因子：
12.7
作者：
Bibby, David F.;McElarney, Iain;Clark, Duncan A.
通讯作者：
Clark, Duncan A.
影响因子：
9.4
作者：
Ginevra, C;Barranger, C;Grattard, F
通讯作者：
Grattard, F
影响因子：
3.1
作者：
Jokela P;Piiparinen H;Mannonen L;Auvinen E;Lappalainen M
通讯作者：
Lappalainen M
影响因子：
9.4
作者：
Babady, N. Esther;Mead, Peter;Kamboj, Mini
通讯作者：
Kamboj, Mini