Simultaneous generation of multiple aqueous droplets in a microfluidic device.

Simultaneous generation of multiple aqueous droplets in a microfluidic device.
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DOI:
10.1016/j.aca.2008.10.009
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发表时间:
2008-12-23
影响因子:
6.2
通讯作者:
Chiu, Daniel T.
Chiu, Daniel T.
中科院分区:
化学1区
文献类型:
--
作者:
Lorenz, Robert M.;Fiorini, Gina S.;Jeffries, Gavin D. M.;Lim, David S. W.;He, Mingyan;Chiu, Daniel T.

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本文描述了一种微流体平台,用于按需生成具有不同化学含量或化学浓度的多个水性液滴,用于基于液滴的实验。这种生成技术是作为对现有的连续流(流动)和离散液滴生成技术的补充而开发的,其通过使得能够同时形成多个离散液滴来实现。在这里,可以产生具有不同化学含量的液滴组,而不会由于每个供应入口的隔离性质而产生交叉污染的风险。使用的压力脉冲产生液滴在平行的描述,液滴尺寸的影响进行检查的情况下,流速和表面活性剂浓度。为了说明这种技术,生成了不同的含染料液滴的阵列,以及显示荧光染料浓度梯度的一组液滴。
This paper describes a microfluidic platform for the on-demand generation of multiple aqueous droplets, with varying chemical contents or chemical concentrations, for use in droplet based experiments. This generation technique was developed as a complement to existing techniques of continuous-flow (streaming) and discrete droplet generation by enabling the formation of multiple discrete droplets simultaneously. Here sets of droplets with varying chemical contents can be generated without running the risk of cross contamination due to the isolated nature of each supply inlet. The use of pressure pulses to generate droplets in parallel is described, and the effect of droplet size is examined in the context of flow rates and surfactant concentrations. To illustrate this technique, an array of different dye-containing droplets was generated, as well as a set of droplets that displayed a concentration gradient of a fluorescent dye.
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