Novel nanostructure-coupled biosensor platform for one-step high-throughput quantification of serum neutralizing antibody after COVID-19 vaccination.
Novel nanostructure-coupled biosensor platform for one-step high-throughput quantification of serum neutralizing antibody after COVID-19 vaccination.
复制标题
新型纳米结构耦合生物传感器平台，用于对 COVID-19 疫苗接种后血清中和抗体进行一步高通量定量
DOI：
10.1016/j.bios.2021.113868
复制
发表时间：
2022-03-01
影响因子：
12.6
通讯作者：
Liu GL
中科院分区：
文献类型：
--
作者：
Huang L;Li Y;Luo C;Chen Y;Touil N;Annaz HE;Zeng S;Dang T;Liang J;Hu W;Xu H;Tu J;Wang L;Shen Y;Liu GL
COVID-19疫苗效率取决于对严重急性呼吸综合征2（SARS-COV-2）峰值尖峰蛋白的中和抗体（NAS）的血清水平（NAS）。和评估开发了一种新型的纳米质免疫吸附测定（Nanopisa）平台，用于在临床血清样品中快速量化SARS-COV-2 NAS NAS，以高通量评估COVID-19，可通过使用Nanoporov hollow cov的nanopiss-limimect-limimect-limimect-limimect-limimect-limimect-limimect-limimect-limimect-limit ottercov syct-limim cot ottercov syct sans sars sars sars sars sars sars。一步临床标本中SARS-COV-2 NA检测的纳米异常可以与金标准的Seronetralization检验和替代病毒中和替代酶联的免疫吸收测定法相当，这是一种快速和高度介绍的NAanopisa vercative-naopisa vercation-naopisa coviens-naopisa corvientive-naopisa corvientive-naopisa corvientived-naopisa coviens-naopisa coviensive-5
COVID-19 vaccination efficacy depends on serum levels of the neutralizing antibodies (NAs) specific to the receptor-binding domain of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. Therefore, a high-throughput rapid assay capable of measuring the total SARS-CoV-2 NA level is urgently needed for COVID-19 serodiagnosis, convalescent plasma therapy, vaccine development, and assessment. Here, we developed a novel nanoplasmonic immunosorbent assay (NanoPISA) platform for one-step rapid quantification of SARS-CoV-2 NAs in clinical serum samples for high-throughput evaluation of COVID-19 vaccine effectiveness. The NanoPISA platform enhanced by the use of nanoporous hollow gold nanoparticle coupling was able to detect SARS-CoV-2 NAs with a limit of detection of 0.2 pM within 15 min without washing steps. The one-step NanoPISA for SARS-CoV-2 NA detection in clinical specimens yielded good results, comparable with those obtained in the gold-standard seroneutralization test and the surrogate virus-neutralizing enzyme-linked immunosorbent assay. Collectively, the one-step NanoPISA might be a rapid and high-throughput NA-quantification platform for evaluating the effectiveness of COVID-19 vaccines.
登录
查看更多内容
影响因子：
4.1
作者：
Huang, Chao;Wen, Tian;Jiao, Yong-Jun
通讯作者：
Jiao, Yong-Jun
影响因子：
56.9
作者：
Kissler, Stephen M.;Tedijanto, Christine;Lipsitch, Marc
通讯作者：
Lipsitch, Marc
通过纳米等离子体彩色成像检测人唾液中的 C 反应蛋白
影响因子：
2.9
作者：
Hu, Wenjun;Dang, Tang;Liu, Gang Logan
通讯作者：
Liu, Gang Logan
影响因子：
12.8
作者：
Kannan SR;Spratt AN;Cohen AR;Naqvi SH;Chand HS;Quinn TP;Lorson CL;Byrareddy SN;Singh K
通讯作者：
Singh K
影响因子：
12.6
作者：
Cetin, Arif E.;Topkaya, Seda Nur
通讯作者：
Topkaya, Seda Nur